白内障超声乳化人工晶状体植入术患者泪液中TXNIP、TREM-1以及bFGF mRNA表达水平及与术后干眼的关系OA

Objective To explore the changes in the mRNA expression of thioredoxin-interacting protein(TXNIP),triggering receptor expressed on myeloid cells-1(TREM-1),and basic fibroblast growth factor(bFGF)in the tears of pa-tients undergoing phacoemulsification and intraocular lens implantation(Phaco+IOL)for cataract(CAT),and their rela-tionship with postoperative dry eye(DE).Methods A total of 118 CAT patients who received Phaco+IOL in our hospital from July 2022 to October 2025 were selected as the CAT group.The patients were assigned to the DE group(40 patients)and the non-DE group(78 patients)based on whether DE occurred in the operated eye.During the same period,59 healthy volunteers with matching demographic characteristics were recruited as the control(Ctrl)group.The mRNA levels of TX-NIP,TREM-1,and bFGF in the tears of the patients were compared.LASSO-Logistic regression was used to explore the in-fluencing factors of DE in CAT patients after surgery.The receiver operating characteristic curve was used to analyze the predictive value of TXNIP,TREM-1,and bFGF mRNA levels for the occurrence of DE in CAT patients after surgery.Internal validation was conducted using the Bootstrap method(1 000 repetitions).Results Compared with the Ctrl group,the mRNA levels of TXNIP,TREM-1 and bFGF in the tears of patients in the CAT group were higher(all P＜0.05).Compared with the non-DE group,the DE group had higher meibomian gland function scores,higher proportions of meibomian lipid viscosity grade＞2,higher proportions of conjunctival relaxation grade 3,and increased mRNA levels of TXNIP,TREM-1,and bFGF in tears(all P＜0.05).Meibomian gland function scores,TXNIP,TREM-1,and bFGF mRNA levels were the influ-encing factors of DE in CAT patients after surgery(all P＜0.05).The area under the curve of TXNIP,TREM-1,and bFGF mRNA levels alone,as well as their combination for predicting DE in CAT patients after surgery was 0.868,0.851,0.877,and 0.950,respectively.The combined prediction was superior to each individual prediction(all P＜0.05).The sensitivities of the TXNIP,TREM-1,and bFGF mRNA levels,as well as their combination for predicting postoperative DE in CAT patients were 80.00％,92.50％,77.50％,and 85.00％,respectively,and the specificities were 85.90％,74.36％,87.18％,and 93.59％,respectively.In addition,the Hosmer-Lemeshow test indicated that the model fitted well,confirming good consis-tency between the predicted probability and the measured results.Conclusion The mRNA levels of TXNIP,TREM-1,and bFGF in the tears of CAT patients increase,and the combination of the three indicators has a relatively high efficiency in predicting postoperative DE in patients.