基于正交试验设计的金花茶扦插生根优化与因素分析OA

[Objective]The study aimed to systematically analyze the effects of substrate type and ratio,hormone type and ratio,hormone con-centration,and treatment method on rooting of cuttings of different Camellia chrysantha varieties using an orthogonal experimental design and establish optimal combinations for each variety,providing theoretical basis for efficient propagation techniques of C.chrysantha.[Method]Using C.nitidissima,C.chuangtsoensis and C.rostrata as materials,a four-factor three-level orthogonal experimental design was adopted to in-vestigate the effects of substrate type and ratio[yellow soil,yellow soil+river sand(1∶1),yellow soil+river sand+peat(3∶1∶1)],hor-mone type and ratio[indole-3-acetic acid(IAA),indole butyric acid(IBA),IAA+IBA(1∶1)],hormone concentration(100,500,1000 mg/L),and treatment methods(quick dip,60 minutes soaking,irrigation)on the survival rate,mean root number,mean root length,and root activity of different C.chrysantha varieties.The main influencing factors affecting the comprehensive cutting performance of different C.chry-santha varieties were identified through orthogonal experimental analysis,and the optimal combinations for each varieties were screened using membership function analysis and range(R)analysis.[Result]For C.nitidissima,factors that influencing the comprehensive cutting perform-ance were in the order of hormone concentration(R=0.44)>substrate type and ratio(R=0.23)>treatment methods(R=0.18)>hormone type and ratio(R=0.16);the optimal combination for enhancing comprehensive rooting performance was yellow soil+river sand(1∶1)substrate,IBA100 mg/L,and 60 min soaking.For C.chuangtsoensis,factors that influenced the comprehensive cutting performance were in the order of treatment methods(R=0.36)>substrate type and ratio(R=0.35)>hormone concentration(R=0.23)>hor-mone type and ratio(R=0.17);the optimal combination for enhancing comprehensive rooting performance was yellow soil+river sand+peat(3∶1∶1)substrate,IAA+IBA(1∶1)100 mg/L,and 60 min soaking.For C.rostrata,factors that influencing the comprehensive cutting performance were in the order of substrate type and ratio(R=0.52)>hormone concentration(R=0.29)>treatment methods(R=0.18)>hormone type and ratio(R=0.05);the optimal for enhancing comprehensive rooting performance was yellow soil+river sand+peat(3∶1∶1)substrate,IAA100 mg/L,and60 minutes soaking.[Conclusion]The main factors affecting the comprehensive cutting perform-ance of rooting for different C.chrysantha varieties are markedly different.Appropriate substrate type and ratio and treatment methods can pro-duce synthetic enhancement.Hormone of low concentration has advantage on promoting rooting of C.chrysantha cuttings.