加拿大披碱草AP2/ERF基因家族的生物信息学分析及干旱胁迫下表达分析OA

[Objective]The study aims to explore the response of the AP2/ERF gene family to drought stress in Elymus canadensis L.[Meth-od]Based on transcriptome sequencing data,genome-wide identification and bioinformatic analysis of the AP2/ERF gene family in Elymus canadensis L.were performed.The expression patterns of differentially expressed AP2/ERF genes under drought stress were further investiga-ted.[Result]The results showed that 45 EcAP2/ERF genes were identified in E.canadensis.Their coding region sequences ranged from 288 to 1302 bp in length,their protein sequences ranged from99 to 395 amino acids(aa)in length,their protein molecular weights ranged from 46 463.55 to10 762.33 Da,and their theoretical isoelectric points ranged from4.20 to11.37.Physicochemical property analyses revealed that 23 EcAP2/ERFs were basic proteins,22 were acidic proteins,2 were unstable proteins,and 43 were stable proteins.All proteins were hy-drophobic and primarily localized in the nucleus.The expression of 6 ECAP2/ERF family genes increased in response to drought stress,sug-gesting they may be key genes in the response to drought stress.[Conclusion]The results of the study can provide a reference for further re-search on drought stress response of AP2/ERF gene family members in E.canadensis.