甘蓝型油菜BnBAM基因家族鉴定与表达分析OA

[Objective]Starch is the most prevalent form of energy storage in plants,found in diverse plant tissues and during various develop-mental stages.Beta-amylase(BAM)is one of the key enzymes involved in starch degradation,playing a widespread role in a series of physio-logical and biochemical processes such as plant growth and development,as well as responses to stress.A genome-wide identification of the BAM gene family in Brassica napus was conducted,alongside investigations into its gene structure,phylogenetic evolution and tissue expres-sion patterns.The aim of the study is to elucidate the roles of family members in B.napus growth,development and stress response processes.[Method]A comprehensive bioinformatics-driven identification and systematic annotation of the β-amylase(BAM)gene family was conduc-ted on the whole-genome dataset of B.napus L.This was achieved through an integrated multi-omics analytical framework,encompassing hid-den markov model(HMM)-based conserved domain screening,phylogenetic reconstruction,conserved motif profiling and chromosomal locali-zation analysis.This approach facilitated the screening and confirmation of members of the β-amylase(BAM)gene family,followed by sys-tematic identification and comprehensive annotation.[Result]A systematic phylogenetic analysis was conducted,which resulted in the classi-fication of these members into four subfamilies.The 31 BnBAM genes were distributed across 15 chromosomes,encoding hydrophilic proteins ranging from 223 to 700 amino acids in length.In the context of cis-element prediction,BAM genes exhibited a pronounced tendency to cluster around MeJA,light response,anaerobic induction factor and ABA cis-elements.Co-linearity analysis revealed 26 interspecies co-linearity rela-tionships between rapeseed BAM genes and nine AtBAM genes from the genus Arabidopsis,along with 41 intraspecies co-linearity relation-ships.Protein interaction analysis revealed 15 interactions among members of the BnBAM gene family.Expression levels of BnBAM family members exhibited variation across different tissues and developmental stages.Notably,BnBAM23 and BnBAM30 demonstrated negligible ex-pression.In the context of diverse abiotic stress conditions and Sclerotinia sclerotiorum infection treatments,BnBAM1,BnBAM2,BnBAM4,Bn-BAM6,BnBAM7,BnBAM10,BnBAM16,BnBAM17,BnBAM20,BnBAM26,BnBAM27 and BnBAM31 demonstrated comparatively elevated levels of activity.[Conclusion]A systematic analysis is conducted to identify 31 members of the BAM gene family in B.napus,and their re-sponse patterns under drought,salinity,low temperature,high temperature and pathogen infection conditions are examined.These findings pro-vide new avenues for research into the functional diversity of BAM genes in B.napus and their potential roles in stress adaptation.