戌时摩腹对肾不藏志型不寐大鼠睡眠-觉醒节律的影响OA

Objective It is to investigate the effects and mechanism of abdominal massage at the Xu period(19:00-21:00)on sleep-wake rhythms in insomnia rats with kidney not storing the emotion.Methods Forty male SD rats were randomly di-vided into normal group,insomnia group,abdominal massage group Ⅰ and abdominal massage group Ⅱ,with 10 rats in each group.The rats of all groups except for the normal group were prepared with subcutaneous injection of D-galactose combined with intraperitoneal injection of 4-chloro-DL-phenylalanine(PCPA)to establish models of insomnia with kidney not storing the emotion.After modeling,the rats of the normal and insomnia groups were treated with daily restraint stress,while the ones of the abdominal massage group Ⅰ and abdominal massage group Ⅱ were treated with abdominal massage daily at the Xu period(19:00-21:00),additionally,the abdominal massage groupⅡwas given Suvorexant solution 10 mg/kg via gavage 2 hours before each abdominal massage.All the groups were treated once daily,continuously treated for 14 days.The daytime spontaneous activity of rats in each group were compared,the levels of monoamine neurotransmitters do-pamine(DA),norepinephrine(NE)and 5-hydroxytryptamine(5-HT)in hypothalamic tissue were measured by ELISA,and the mRNA and protein expressions of orexin A(OXA),cAMP response element-binding protein(CREB),brain and muscle ARNT-like-1(BMAL1)and period circadian protein 1(PER1)in hypothalamic tissue were detected by real-time quantitative PCR and Western blot techniques.Results Compared with the control group,the activity distance and duration ofrats in the insomnia group were significantly increased and their resting time was significantly decreased at all daytime time points(all P<0.05);the levels of DA and NE,as well as the relative mRNA and protein expressions of OXA,CREB and BMAL1 in hypothalamic tissue were all significantly increased(all P<0.05),while the level of 5-HT and the relative mRNA and protein expressions of PER1 were all significantly reduced(all P<0.05).Compared with the insomnia group,the activity distance(except for 11:00 in the abdominal massage group Ⅰ)and duration of rats in the abdominal massage group Ⅰ and abdominal massage group Ⅱ were significantly decreased and their resting times were significantly decreased at all daytime time points(all P<0.05);the levels of DA and NE,as well as the relative mRNA and protein expressions of OXA,CREB and BMAL1 in hypothalamic tissue were all significantly decreased(all P<0.05),while the level of 5-HT and the relative mRNA and protein expressions of PER1 were all significantly increased(all P<0.05).The relative mRNA and protein expressions of OXA,CREB and BMAL1 in hypothalamic tissue of rats in the abdominal massage group Ⅱ were all significantly lower,while the relative protein expression of PER1 was all significantly higher than those in the abdominal massage group Ⅰ(all P<0.05).Conclusion Abdominal massage at the Xu period(19:00-21:00)can regulate the spontaneous activity pattern and sleep-wake rhythms in insomnia rats with kidney not storing the emotion,and its mechanism may be related to influence the levels of neurotransmitters involved in sleep homeostasis and the hypothalamic OXA/CREB/PER1 signaling pathway.