金骨莲胶囊对绝经后骨质疏松模型大鼠骨组织结构的影响OA

BACKGROUND:Numerous studies have shown that Jingulian capsule can down-regulate the levels of inflammatory factors in vivo,and inflammatory factors such as interleukin-1β,interleukin-6 and tumor necrosis factor-α play an important role in the pathogenesis of osteoporosis. OBJECTIVE:To study the effect of Jingulian capsule on bone tissue reconstruction after osteoporosis,and to provide theoretical basis for the feasibility of promoting the application of traditional Chinese medicine in the treatment of osteoporosis patients in the future. METHODS:Sixty-three 12-week-old female Sprague-Dawley rats were randomly divided into three groups:blank group(n=7),sham-operation group(n=7),and model group(n=42).In the sham-operation group,only part of the fat around the ovary was removed,while the model group underwent bilateral ovariectomy to establish a postmenopausal osteoporosis model.Three months after modeling,blank group rats(n=7),as well as seven rats each from the sham-operation group and model group,were sacrificed to detect femoral and tibial bone mineral density,serum estradiol level,and histopathology.After successful modeling,the remaining 35 model rats were randomly divided into model control group,western medicine group,Chinese medicine low-dose group,Chinese medicine medium-dose group,and Chinese medicine high-dose group,with 7 rats in each group.The Chinese medicine low-,medium-,and high-dose groups were intragastrically administered Jingulian capsule at 0.787 5,1.575,and 3.15 g/(kg-d)(5,10,and 20 times the clinical equivalent dose),respectively.The western medicine group received alendronate sodium 0.167 mg/(kg.d).The sham-operation group and model control group received an equal volume of distilled water.After 3 months of gavage,bone mineral density,serum estradiol,alkaline phosphatase,acid phosphatase,interleukin-1β,interleukin-6,and tumor necrosis factor-α levels were measured in each group,and tibial histomorphological observation and tibial CT scanning were performed. RESULTS AND CONCLUSION:(1)Three months after modeling,serum estradiol level,femoral bone mineral density,and tibial bone mineral density in the model group were significantly lower than those in the sham-operation group and blank group(P＜0.05).Hematoxylin-eosin staining showed sparse tibial trabeculae,wide trabecular spacing,disordered structure,and increased adipocytes,indicating that bilateral ovariectomy successfully reduced estrogen secretion and established a postmenopausal osteoporosis model.(2)After 3 months of gavage,serum estradiol levels in the Chinese medicine high-,medium-,and low-dose groups and the sham-operation group were higher than those in the model control group(P＜0.05),indicating that Jingulian capsule up-regulated serum estradiol level.(3)Serum tumor necrosis factor-α,interleukin-1β,and interleukin-6 levels in the Chinese medicine high-,medium-,and low-dose groups and the sham-operation group were lower than those in the model control group(P＜0.01).Acid phosphatase activity in the sham-operation group and all Chinese medicine dose groups was significantly lower than that in the model control group(P＜0.05).Femoral and tibial bone mineral density in the sham-operation group,western medicine group,and Chinese medicine high-and medium-dose groups was significantly higher than that in the model control group(P＜0.05).Micro-CT and hematoxylin-eosin staining results showed that the model control group had sparse tibial trabeculae,increased spacing,and loose structure,while the bone structure was significantly improved in all Chinese medicine dose groups.To conclude,bilateral ovariectomy in rats leads to decreased estrogen,increased inflammatory factor levels,and decreased bone mineral density.The western medicine alendronate sodium can improve osteoporosis.Jingulian capsule can effectively down-regulate the levels of interleukin-1β,interleukin-6,and tumor necrosis factor-α,increase bone mineral density,repair and reconstruct osteoporotic bone tissue structure,and improve osteoporosis in rats.