黄芩汤含药血清通过AGR2-WNT信号轴干预结肠癌细胞增殖和干细胞特性的作用机制研究OA

Objective To investigate the intervening effects of Huangqin Decoction(黄芩汤,HQD)medicated serum on the prolifera-tion and stemness of colon cancer cells and the underlying molecular mechanisms.Methods Wistar rats were orally administered HQD at crude drug doses of 20 g/kg for 7 consecutive days to prepare gradient concentrations(5％,10％,and 20％)of HQD medicated serum.Transwell assays were performed to detect colon cancer cell migration and invasion.Spheroid formation assays were used to mea-sure the diameter and number of colon cancer stem cell spheroids,while Cell Counting Kit-8(CCK-8)assay was employed to assess their proliferation.The protein expression levels of anterior gradient 2(AGR2),β-catenin,and Wnt family member 3A(Wnt3a)were detected by Western blot(WB).The relative mRNA expression levels of AGR2,β-catenin,and WNT3A were measured by qRT-PCR.Results Compared with the control group,the medium/high-concentration HQD medicated serum groups showed significantly reduced numbers of migrated and invaded colon cancer cells(P<0.01).Both the number and diameter of colon cancer stem cell spheroids were significantly decreased(P<0.01).Furthermore,the protein expression levels of AGR2 and Wnt3a and the mRNA expression levels of AGR2 and WNT3A were significantly downregulated in these cells(P<0.01).Conclusion HQD medicated serum may inhibit the prolif-eration,migration,and stemness of colon cancer cells.This therapeutic effect is likely mediated by suppressing the overexpression of Wnt3a and AGR2 within the AGR2-WNT signaling axis,thereby restoring disrupted stem cell homeostasis.