加味四君子汤调控PI3K/AKT/FoxO1信号通路对CTX诱导卵巢早衰大鼠的作用机制研究OA

Objective To investigate the mechanism by which Jiawei Sijunzi Decoction(加味四君子汤,JSD)alleviates cyclophospha-mide(CTX)-induced premature ovarian failure(POF)in rats through regulation of the PI3K/AKT/FoxO1 signaling pathway.Methods Fifty rats were numbered and randomly divided into a blank group(n=10)and a model control group(n=40).The POF model was estab-lished by intraperitoneal injection of CTX.After successful modeling,the model rats were randomly divided into model group,low/medium/high-dose JSD groups(n=10 each).Serum levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),anti-Müllerian hormone(AMH),and gonadotropin-releasing hormone(GnRH)were measured by ELISA.Ovarian tissue mor-phology was observed by HE staining.Protein expression levels of PI3K,AKT,FoxO1,Bcl-2,Caspase-3,and Bax in ovarian tissues were detected by Western blot(WB).mRNA expression levels of PI3K,AKT,and FoxO1 in ovarian tissues were detected by q-PCR.Results Compared with the blank group,the number of atretic follicles,serum FSH and LH levels,as well as the protein and mRNA expression levels of PI3K,p-PI3K,AKT,p-AKT,Bax,and Caspase-3 in the model group were significantly increased(all P＜0.05),while serum E2 and AMH levels,and the protein and mRNA expression levels of Bcl-2 and FoxO1 were significantly decreased(all P＜0.05).Compared with the model group,the above indexes in the JSD dose groups showed the opposite trend(all P＜0.05).Conclusion JSD may alleviate CTX-induced premature ovarian failure by regulating the PI3K/AKT/FoxO1 signaling pathway,ameliorating ovarian tissue morphology and reducing excessive follicular atresia and depletion.