炙红芪调节脾气虚大鼠肠道免疫的作用机制研究OA

Objective To investigate the mechanism of honey-processed Radix Hedysari(HRH)on intestinal immunity in rats with spleen qi deficiency(SQD).Methods The SQD rat model was established using a compound modeling method involving excessive fatigue,restricted diet,and purgation with bitter-coldherbs.HE staining was employed to observe the pathological damage of colonictis-sue in each group;ELISA was used to detect the expression levels of SIgA,CXCL1,and CXCL2 in colonic tissue homogenate;PCR was applied to determine the relative expression of IL-4 mRNA,IL-10 mRNA,IL-12 mRNA,PPARγ mRNA and TNF-α mRNA in colonic tissue of rats in each group.Results Compared with the blank group,the model group showed a significant decrease in the expression of colonic SIgA and the relative expression levels of IL-4 mRNA,IL-10mRNA,PPARγ mRNA,(P＜0.01),while theexpression levels of CXCL1,CXCL2,IL-12 mRNA,and TNF-α mRNA were significantly increased(P＜0.01).Compared with the model group,HRH could significantly increase the expression of colonic SIgA,IL-4 mRNA,IL-10 mRNA,and PPARγ mRNA in rats with SQD(P＜0.01),and inhibit the expression of CXCL1,CXCL2,IL-12 mRNA,and TNF-α mRNA(P＜0.05 or P＜0.01).Conclusion HRH can upregulate the level of SIgA in colonic tissue of rats with SQD,promote the relative expression levels of IL-4 mRNA,IL-10 mRNA,and PPARγ mRNA,thereby improving the intestinal immune function of rats with spleen qi deficiency.