祛白酊剂调控NLRP3/Caspase-1/GSDMD通路介导的细胞焦亡治疗白癜风小鼠的机制研究OA

Objective To investigate the effect and mechanism of Qubai Tincture(祛白酊剂,QBT)on NLRP3/caspase-1/GSDMD pathway-mediated pyroptosis in a mouse model of vitiligo.Methods Thirty-six C57BL/6 male mice were randomly divided into a blank control group,a model group,a positive control group,and low-/medium-/high-dose QBT groups.After successful modeling,except for the blank and model groups,the other groups were treated topically with tacrolimus ointment or QBT for 2 weeks.Changes in skin appearance were observed and depigmentation scores were recorded.Pathological changes in skin tissue were examined via HE stain-ing.Serum levels of IL-6,IL-18,IL-1β,TNF-α,TYR,and SOD were measured by ELISA.The mRNA and protein expression levels of NLRP3,Caspase-1,and GSDMD in skin tissues were detected using RT-PCR and Western blot(WB),respectively.Results Com-pared with the blank group,the model group exhibited whitened fur,thickened epidermis,reduced melanin,elevated levels of inflam-matory factors,and significantly increased mRNA and protein expression of NLRP3,Caspase-1,and GSDMD.After intervention with QBT,the mice showed improved fur color,increased melanin content,decreased levels of inflammatory factors,and reduced expression of molecules related to the NLRP3/caspase-1/GSDMD pathway,all in a dose-dependent manner.The effect in the high-dose QBT group was close to that in the positive control group.Conclusion QBT may inhibit pyroptosis and alleviate the inflammatory response by regu-lating the NLRP3/caspase-1/GSDMD pathway,thereby promoting melanogenesis in the skin of the vitiligo model mice.