丙泊酚改善小鼠肺部辐射暴露引起的造血系统损伤实验研究OA

Objective:This study aimed to investigate the long-term effects of propofol on the hematopoietic sys-tem in mice with localized lung irradiation and to evaluate whether propofol has a protective role against such dam-age.Methods:A localized lung irradiation model was established in mice,with control,irradiation,control+propo-fol,and irradiation+propofol groups set up.From 1 to 6 months after irradiation,the peripheral blood cell counts,lymphocyte-to-myeloid cell ratios were dynamically monitored;the proportions of lymphocytes,myeloid cells,and hematopoietic stem/progenitor cells(including LSK,CD34-LSK,and CD34-LSK cells)in the bone marrow were ana-lyzed;and the clonogenic capacity of bone marrow cells was assessed via the granulocyte-macrophage colony-forming unit(CFU-GM)assay.Results:Lung irradiation led to persistent hematopoietic system abnormalities in mice,inclu-ding:abnormal increases in peripheral blood white blood cell,red blood cell counts,and neutrophil percentage,with a decrease in lymphocyte percentage(all P＜0.05);a myeloid skewing in both peripheral blood and bone marrow,characterized by increased myeloid cell proportions and decreased B and T lymphocyte proportions(all P＜0.05);ab-normal increases in the proportions of hematopoietic progenitor cells and LSK cells in the bone marrow,with early expansion of more primitive CD34-LSK cells and early reduction of CD34-LSK cells(all P＜0.05);and long-term impairment of bone marrow cell CFU-GM formation capacity(P＜0.05).Propofol treatment significantly reversed the elevated peripheral blood cell counts and the lymphoid-to-myeloid skewing(all P＜0.05);corrected the imbal-anced proportions of hematopoietic stem/progenitor cell populations in the bone marrow;and significantly restored the impaired clonogenic capacity of bone marrow cells(P＜0.05).Conclusion:Localized lung irradiation can cause persistent and extensive damage to the hematopoietic system in mice,involving mature blood cells,differentiation pathways,and hematopoietic stem/progenitor cell functions.Propofol can effectively alleviate these damages,sugges-ting that it exerts its effects by modulating the hematopoietic microenvironment and maintaining stem cell homeosta-sis.This study provides important preclinical evidence for the potential use of propofol as a radioprotector to mitigate hematopoietic system complications associated with thoracic radiotherapy.