苦荞醋饮联合沙棘醋饮对代谢相关脂肪性肝病斑马鱼幼鱼脂质代谢、氧化应激及炎症反应的调控作用OA

Objective:To explore the regulatory effects of bitter buckwheat vinegar drink combined with sea buckthorn vinegar drink on lipid metabolism disorder,oxidative stress and inflammatory state in zebrafish larvae with metabolism-related fatty liver disease(MAFLD).Methods:Taking wild-type juvenile zebrafish as the subjects,they were randomly divided into the blank control group,the model group,the buckwheat vinegar drink group,the sea buckthorn vinegar drink group and the combined intervention group.After continuous administration for 3 weeks,the survival rate,total cholesterol(TC),triglyceride(TG),and malondialdehyde(MDA)levels of each group were detected.The lipid deposition in the liver and histopathological changes were observed,and the expression of lipid metabolism and inflammation-related genes was analyzed.Results:Compared with the model group,vinegar drink intervention could increase the survival rate of MAFLD zebrafish larvae.No significant improvement was observed in liver HE staining and oil red O staining.However,molecular-level indicators confirmed that vinegar drink intervention triggered compensatory upregulation of CYP51,among which bitter buckwheat vinegar drink significantly down-regulated SREBF1 expression and reduced total cholesterol(TC)content.Vinegar drink intervention effectively activated the Nrf2 pathway,down-regulated the production of MDA,and significantly inhibited the expression of IL-6,TNF-α and IL-8.Conclusions:Buckwheat vinegar drink,sea buckthorn vinegar drink and their combined vinegar drink intervention may exert liver-protective regulatory effects on MAFLD at the molecular level by regulating cholesterol synthesis,antioxidation and anti-inflammation.However,it has not been confirmed that they can improve liver histopathological damage,and the related mechanisms still need further study.