补阳还五汤抗肺纤维化的作用机制研究OACHSSCD

Objective:To investigate the effect of Buyang Huanwu Decoction-containing serum on the anti-pulmonary fibrosis effect of the long non-coding RNA-plasmacytoma variant translocation gene 1(lnc-PVT1)/Janus kinase(JAK)/signal transducer and ac-tivator of transcription 3(STAT3)signaling pathway in mouse lung fibroblasts.Methods:Mouse lung fibroblasts were divided into five groups:normal group,model group,small interfering RNA targeting lnc-PVT1(siPVT1)+model group,Buyang Huanwu Decoc-tion-containing serum+model group,and siPVT1+Buyang Huanwu Decoction-containing serum+model group.Cell viability,cell migration,and cell cycle were assessed,and the levels of interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α (TNF-α)in the cell culture supernatant were measured.The expression of genes and proteins related to the signaling pathway and collagen synthesis was also detected.Results:Compared with the model group,the migration speed of mouse lung fibroblasts was significantly decreased in all groups containing Buyang Huanwu Decoction(P＜0.01),cell proliferation was decreased(P＜0.01),the proportion of cells in the G1 phase was significantly increased(P＜0.01),and the proportions of cells in the S and G2 phases were significantly decreased(P＜0.01).In the Buyang Huanwu Decoction group,the levels of inflammatory mediators in the cul-ture supernatant of mouse lung fibroblasts were significantly decreased(P＜0.01).Compared with the model group,the relative ex-pression levels of JAK,α-smooth muscle actin(α-SMA),collagen Ⅰ(Collagen Ⅰ),and lnc-PVT1 mRNA and protein were signifi-cantly decreased(P＜0.01).Conclusion:Buyang Huanwu Decoction may alleviate pulmonary fibrosis by inhibiting lnc-PVT1 and activating the JAK/STAT signaling pathway,thereby reducing the proliferation and activation of lung fibroblasts and the deposition of extracellular matrix such as collagen.