结合灰色关联度优化鲜竹沥总酚酸的纯化工艺及其体外活性分析OACHSSCD

Objective:To investigate the purification process of total phenolic acids from Succus Bambusae using macroporous ad-sorption resins and evaluate the in vitro antibacterial activity of the purified total phenolic acid fraction.Methods:Macroporous ad-sorption resins with different polarities were screened using grey relational analysis.A single-factor experiment was conducted to op-timize the loading volume,adsorption time,washing volume,ethanol elution volume fraction,and elution volume of Succus Bambus-ae.The total phenolic acid content of the purified and enriched freeze-dried product was determined,and its components were quali-tatively analyzed by GC-MS.The diameters of the inhibition zones produced by different concentrations of total phenolic acids a-gainst Staphylococcus aureus and Pseudomonas aeruginosa were determined.Results:Grey relational analysis showed that the AB-8 macroporous adsorption resin had the highest degree of correlation(0.91).The optimal purification process was as follows:a loading volume of 3.5 BV(43 mL),an adsorption time of 30 min,a washing volume of 5 BV,and elution with 35 mL of 70％ethanol.The antibacterial activity of the purified total phenolic acid fraction of Succus Bambusae against the two tested bacteria increased with increasing concentration.Conclusion:The optimized purification process is stable and feasible and can be used for the purification of total phenolic acids from Succus Bambusae.The purified product exhibits good antibacterial activity.