黑老虎根挥发油抗氧化活性部位筛选及化学成分解析OACHSSCD

Objective:To screen the antioxidant active fractions of the volatile oil from Kadsurae Coccineae Radix and analyze its chemical composition.Methods:The volatile oil of Kadsurae Coccineae Radix was extracted using seven methods.The antioxidant active fractions were evaluated by in vitro antioxidant assays,and their antioxidant chemical composition was analyzed using gas chromatography-mass spectrometry(GC-MS).Results:The antioxidant capacities of the volatile oil extracts of Kadsurae Coccineae Radix were ranked as follows:anhydrous ethanol extract＞methanol extract＞n-butanol extract＞ethyl acetate extract,while the an-tioxidant capacities of the n-hexane,petroleum ether,and dichloromethane extracts were relatively weak.A total of 41,38,42,49,44,41,and 40 compounds were identified in the volatile oil extracts of Kadsurae Coccineae Radix obtained using n-hexane,petrole-um ether,dichloromethane,ethyl acetate,n-butanol,anhydrous ethanol,and methanol,respectively.These compounds mainly includ-ed aromatic compounds,fatty compounds,and terpenoids.The common components with relatively high contents in the volatile oil extracts were dimethyl phthalate,methyl tetradecanoate,methyl hexadecanoate,methyl 2-hexylcyclopropaneoctanoate,methyl(Z,Z)-9,12-octadecadienoate,methyl trans-9-octadecenoate,methyl stearate,(Z,Z)-3,13-octadecadien-1-ol,and methyl 20-methylico-sanoate.Conclusion:The volatile oil extract of Kadsurae Coccineae Radix obtained with anhydrous ethanol exhibited the strongest antioxidant capacity.Methyl(Z,Z)-9,12-octadecadienoate,methyl trans-9-octadecenoate,and(Z,Z)-3,13-octadecadien-1-ol were the main components identified;however,the antioxidant capacities of the seven volatile oil extracts differed considerably.