参附注射液对慢性心力衰竭大鼠心肌细胞凋亡的作用机制研究OACHSSCD

Objective:To investigate the effects of Shenfu Injection on cardiomyocyte apoptosis in chronic heart failure(CHF)and provide experimental evidence for drug-targeted therapy of CHF and the rational application of Shenfu Injection.Methods:Forty rats were randomly divided into the control group,model group,metoprolol group,and Shenfu Injection group according to a random number table method,with 10 rats in each group.A CHF rat model was established by intraperitoneal injection of adriamycin.After modeling,the general behavioral signs of rats were observed.Cardiac function was evaluated by echocardiography.Pathological changes in myocardial tissue were observed using hematoxylin-eosin(HE)staining.The protein expression levels of cysteine-aspar-tic acid protease-3(Caspase-3),cysteine-aspartic acid protease-9(Caspase-9),B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X protein(Bax)were detected by Western blot(WB).The contents of N-terminal pro-B-type natriuretic peptide(NT-proBNP),Caspase-3,Caspase-9,and Bax were determined by enzyme-linked immunosorbent assay(ELISA).The mRNA expression levels of Caspase-3,Bcl-2,and Bax were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR).Re-sults:Compared with the model group,the Shenfu Injection group showed improvements in general behavioral signs and pathological morphology,increased left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)(P<0.05),and decreased contents of NT-proBNP,Bax,Caspase-3,and Caspase-9(P<0.05).The mRNA expression levels of Caspase-3 and Bax were decreased(P<0.05).The protein expression levels of Caspase-3,Caspase-9,and Bax were decreased(P<0.05),whereas the protein expression level of Bcl-2 was increased(P<0.05).Conclusion:Shenfu Injection can effectively regulate apoptosis-re-lated factors,inhibit cardiomyocyte apoptosis,and delay the progression of CHF.