巴戟天含药血清抑制前列腺癌PC3细胞侵袭转移的机制研究OACHSSCD

Objective:To investigate the effects of Morinda officinalis-containing serum on epithelial-mesenchymal transition(EMT)in prostate cancer PC3 cells through the protein tyrosine kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:M.officinalis-containing serum was prepared.PC3 cells were divided into the normal group,blank serum group,low-,medium-,and high-dose M.officinalis-containing serum groups(5%,10%,and 20%,respectively),JAK2/STAT3 signaling pathway activator Colivelin group,and high-dose M.officinalis-containing serum combined with Colivelin group.The 5-ethynyl-2'-deoxyuridine(EdU)assay was used to detect cell proliferation.The Transwell assay was used to evaluate cell migration and invasion abilities.Western blot was performed to detect the expression levels of E-cadherin,N-cadherin,vimen-tin,JAK2,phosphorylated JAK2(P-JAK2),STAT3,and phosphorylated STAT3(P-STAT3).Results:Compared with the normal group,the proliferation,migration,and invasion abilities of PC3 cells in the low-,medium-,and high-dose M.officinalis-containing serum groups were decreased.The protein expression levels of vimentin,N-cadherin,P-JAK2,and P-STAT3 were decreased,where-as the protein expression level of E-cadherin was increased.Compared with the high-dose M.officinalis-containing serum group,the proliferation,migration,and invasion abilities of PC3 cells in the high-dose M.officinalis-containing serum combined with Colivelin group were increased.The protein expression levels of vimentin,N-cadherin,P-JAK2,and P-STAT3 were increased,whereas the protein expression level of E-cadherin was decreased.Conclusion:M.officinalis-containing serum can significantly inhibit the pro-liferation,invasion,and metastasis of prostate cancer PC3 cells.Its mechanisms may be related to the regulation of the JAK2/STAT3 signaling pathway to inhibit EMT in PC3 cells.