健脾消癌方对结肠癌HCT116细胞转移的影响及机制研究OACHSSCD
Effects and Mechanisms of Jianpi Xiaoai Prescription in Inhibiting Metastasis of Colorectal Cancer HCT116 Cells
目的:探究健脾消癌方对结肠癌 HCT116 细胞转移的影响及作用机制.方法:观察健脾消癌方对 HCT116 细胞外泌体浓度及外泌体中 miR-21 表达的影响.将细胞随机分为健脾消癌方高、中、低剂量组及对照组.经相应处理后,采用CCK8 实验检测 HCT116 细胞增殖能力,Transwell 实验检测迁移、侵袭能力,蛋白质印迹法实验检测细胞外信号调节激酶(ERK)、磷酸化细胞外信号调节激酶(p-ERK)、信号转导和转录激活因子 3(STAT3)、磷酸化信号转导和转录激活因子 3(p-STAT3)、二酰基甘油激酶 β(DGKB)的蛋白表达水平.结果:健脾消癌方能降低 HCT116 细胞外泌体浓度,且能显著抑制外泌体中 miR-21 的表达(P<0.01).与对照组比较,健脾消癌方高、中、低剂量组在24 h、48 h、72 h 的细胞增殖能力均显著降低(P<0.05);上述时间点的细胞侵袭及迁移率也均低于对照组(P<0.05),且呈剂量依赖性;中、高剂量组的 ERK表达水平低于对照组,低、中、高剂量组的 p-ERK 表达水平低于对照组,中、高剂量组 STAT3 表达水平低于对照组;低、中、高剂量组 p-STAT3 表达水平低于对照组,低、中、高剂量组的 DGKB 表达水平均高于对照组,差异有统计学意义(P<0.05).结论:健脾消癌方可能通过调控 EV/miRNA/DGKB/ERK/STAT3 轴,发挥抑制结直肠癌 HCT116 细胞转移的作用.
Objective:To investigate the effects of Jianpi Xiaoai Prescription on the metastasis of colorectal cancer HCT116 cells and its mechanisms.Methods:The effects of Jianpi Xiaoai Prescription on the concentration of exosomes secreted by HCT116 cells and the expression of miR-21 in exosomes were investigated.The cells were randomly divided into the high-,medium-,and low-dose Jianpi Xiaoai Prescription groups and the control group.After corresponding treatments,the proliferation ability of HCT116 cells was detected using the CCK-8 assay,the migration and invasion abilities were evaluated using the Transwell assay,and the protein ex-pression levels of extracellular signal-regulated kinase(ERK),phosphorylated extracellular signal-regulated kinase(p-ERK),signal transducer and activator of transcription 3(STAT3),phosphorylated signal transducer and activator of transcription 3(p-STAT3),and diacylglycerol kinase β(DGKB)were detected by Western blot.Results:Jianpi Xiaoai Prescription reduced the concentration of exosomes secreted by HCT116 cells and significantly inhibited the expression of miR-21 in exosomes(P<0.01).Compared with the control group,the proliferation ability of cells in the high-,medium-,and low-dose Jianpi Xiaoai Prescription groups was signifi-cantly decreased at 24,48,and 72 h(P<0.05).At the same time points,the cell invasion and migration rates in the high-,medi-um-,and low-dose groups were also lower than those in the control group(P<0.05),showing a dose-dependent trend.The expres-sion level of ERK in the medium-and high-dose groups was lower than that in the control group.The expression level of p-ERK in the low-,medium-,and high-dose groups was lower than that in the control group.The expression level of STAT3 in the medium-and high-dose groups was lower than that in the control group.The expression level of p-STAT3 in the low-,medium-,and high-dose groups was lower than that in the control group.The expression levels of DGKB in the low-,medium-,and high-dose groups were higher than those in the control group.All differences were statistically significant(P<0.05).Conclusion:Jianpi Xiaoai Prescrip-tion may inhibit the metastasis of colorectal cancer HCT116 cells by regulating the EV/miRNA/DGKB/ERK/STAT3 axis.
何威华;吕俊;何兰
武汉市中医医院,武汉,430050武汉市中医医院,武汉,430050湖南中医药大学第一附属医院,长沙,410007
医药卫生
健脾消癌方结直肠癌外泌体增殖侵袭迁移机制EV/miRNA/DGKB/ERK/STAT3 轴
Jianpi Xiaoai PrescriptionColorectal cancerExosomesProliferationInvasionMigrationMechanismEV/miR-NA/DGKB/ERK/STAT3 axis
《世界中医药》 2026 (11)
1991-1998,2005,9
国家自然科学基金项目(82305329)湖北省中医药管理局中医药科研项目(ZY2023F054).
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