蒙药清热八味胶囊退热、抗炎药效学作用的初步评价OACHSSCD
Preliminary Evaluation of the Antipyretic and Anti-inflammatory Pharmacodynamic Effects of Mongolian Medicine Qingre Bawei Capsules
目的:初步评价蒙药清热八味胶囊的退热和抗炎药效,并探讨其退热抗炎机制.方法:以皮下注射干酵母混悬液法建立大鼠发热模型,评价口服清热八味胶囊对大鼠体温的影响,并采用酶联免疫吸附剂测定法(ELISA)检测大鼠血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α),下丘脑中前列腺素 E2(PGE2)、环磷酸腺苷(cAMP)的水平.以涂抹二甲苯致炎法建立小鼠耳肿胀急性炎症模型,评价口服清热八味胶囊对小鼠耳重的影响,并以ELISA 法测定小鼠血清中 IL-1β、IL-6、TNF-α 等炎症介质的水平.结果:皮下注射干酵母混悬液后大鼠体温显著升高(≥0.8℃),发热模型建立成功.与模型组比较,灌胃给药后发热大鼠体温下降(P<0.001),血清中 IL-1β、IL-6、TNF-α炎症介质水平显著降低(P<0.05),下丘脑中 PGE2、cAMP 的水平显著下降(P<0.001).小鼠耳郭涂抹二甲苯后出现明显肿胀,给药组耳肿胀度显著降低(P<0.05),肿胀率下降,血清中 IL-1β、IL-6、TNF-α 炎症介质水平显著降低(P<0.01).结论:清热八味胶囊能通过降低相关炎症介质的表达水平,减轻炎症反应,实现良好的退热抗炎药效,其退热机制是否与抑制 PGE2 通路有关有待进一步深入研究.
Objective:To preliminarily evaluate the antipyretic and anti-inflammatory effects of Mongolian medicine Qingre Bawei Capsules and explore their underlying mechanisms.Methods:A rat fever model was established by subcutaneous injection of dry yeast suspension.The effect of oral administration of Qingre Bawei Capsules on rat body temperature was evaluated.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in rat serum,as well as prostaglandin E2(PGE2)and cyclic adenosine monophosphate(cAMP)levels in the hypothalamus,were determined using enzyme-linked immu-nosorbent assay(ELISA).A mouse acute ear edema inflammation model was established by topical application of xylene.The effect of oral administration of Qingre Bawei Capsules on mouse ear weight was evaluated,and the levels of inflammatory mediators,inclu-ding IL-1β,IL-6,and TNF-α,in mouse serum were measured using ELISA.Results:After subcutaneous injection of dry yeast sus-pension,the body temperature of rats increased significantly(≥0.8℃),indicating successful establishment of the fever model.Compared with the model group,oral administration of Qingre Bawei Capsules decreased the body temperature of febrile rats(P<0.001),significantly reduced the serum levels of inflammatory mediators IL-1β,IL-6,and TNF-α(P<0.05),and markedly de-creased the levels of PGE2 and cAMP in the hypothalamus(P<0.001).Mice exhibited obvious ear swelling after topical applica-tion of xylene,while the ear swelling degree was significantly reduced in the administration group(P<0.05),accompanied by a de-creased swelling rate and significantly reduced serum levels of inflammatory mediators IL-1β,IL-6,and TNF-α(P<0.01).Con-clusion:Qingre Bawei Capsules exert antipyretic and anti-inflammatory effects by reducing the expression levels of related inflam-matory mediators and alleviating inflammatory responses.Whether the antipyretic mechanisms are associated with inhibition of the PGE2 pathway requires further in-depth investigation.
史寒玲;曾鑫;杨建军;王长虹;尉小慧
上海中医药大学中药研究所/中药标准化教育部重点实验室,上海,201210上海中医药大学中药研究所/中药标准化教育部重点实验室,上海,201210内蒙古奥特奇蒙药股份有限公司,乌兰浩特,750336上海中医药大学中药研究所/中药标准化教育部重点实验室,上海,201210上海中医药大学中药研究所/中药标准化教育部重点实验室,上海,201210
医药卫生
清热八味胶囊退热抗炎白细胞介素-1β白细胞介素-6肿瘤坏死因子-α前列腺素 E2环磷酸腺苷
Qingre Bawei CapsulesAntipyreticAnti-inflammatoryInterleukin 1βInterleukin 6Tumor necrosis factor αProstaglandin E2Cyclic adenosine monophosphate
《世界中医药》 2026 (11)
1984-1990,7
国家科技部重点研发计划项目(2022YFC3501702).
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