基于高分辨质谱的海桐皮汤提取膏透皮成分鉴定OACHSSCD

Objective:To analyze the chemical constituents of Haitongpi Decoction Extract Ointment and the transdermal compo-nents after percutaneous administration using ultra-high-performance liquid chromatography coupled with quadrupole/electrostatic field Orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive Orbitrap HRMS).Methods:Twelve male rats were randomly divided into a blank group and a treatment group.Rats in the treatment group received transdermal administration of Haitongpi De-coction Extract Ointment twice daily for 7 consecutive days.Subcutaneous tissues from the administration sites were collected from rats in each group for analysis.Separation was performed using a Phenomenex Kinetex C18 liquid chromatography column with 0.01%acetic acid aqueous solution(A)and isopropanol/acetonitrile(B)as the mobile phase system under gradient elution.Mass spectrometry analysis was performed using an Orbitrap Exploris 120 mass spectrometer,and MS1 and MS2 data were acquired using Xcalibur 4.2 software.The prototype components and transdermal components of Haitongpi Decoction Extract Ointment were identi-fied by comparison with reference standards,literature data,and databases.Results:A total of 123 constituents were identified in Haitongpi Decoction Extract Ointment,including 6 from Zanthoxyli Cortex,11 from Clematidis Intricatae Herba,7 from Olibanum,24 from Myrrha,35 from Angelicae Sinensis Radix,10 from Zanthoxyli Pericarpium,10 from Chuanxiong Rhizoma,46 from Cartha-mi Flos,12 from Clematidis Radix et Rhizoma,10 from Angelicae Dahuricae Radix,41 from Glycyrrhizae Radix et Rhizoma,and 12 from Saposhnikoviae Radix.These constituents included 69 shikimic acid and phenylpropanoid derivatives,22 alkaloids,21 carbo-hydrates,15 polyketides,7 terpenoids,5 fatty acids,and 3 amino acid/short peptide compounds.A total of 31 prototype drug compo-nents were detected in the subcutaneous tissues at the administration sites of rats.Conclusion:High-resolution mass spectrometry was used to identify the chemical constituents of Haitongpi Decoction Extract Ointment and the components migrating into tissues,thereby clarifying the transdermal components of Haitongpi Decoction after percutaneous administration.This study provides prelim-inary evidence for its active components and potential mechanisms of action,and offers insights for the development of topical Chi-nese herbal preparations and modern research on external therapies.