复方金钱草颗粒对去势联合雌激素诱导慢性非细菌性前列腺炎大鼠的治疗作用OA

Objective To investigate the therapeutic effect of Compound Jinqiancao Granules on a rat model of chronic nonbacterial prostatitis(CNP)induced by castration combined with estrogen administration.Methods Sixty healthy male SD rats were randomly divided into six groups using a random number table:blank group,model group,positive control group,and high-,medium-,and low-dose groups of Compound Jinqiancao Granules,with 10 rats in each group.Except for the blank group,all rats in the other groups were subjected to bilateral orchiectomy combined with estrogen injection to establish the CNP model.After successful modeling,the positive control group was intragastrically administered Qianlieshuitong solution at a dose of 0.324 g/kg,and the Compound Jinqiancao Granules groups were treated with 3.24 g/kg,1.62 g/kg,and 0.81 g/kg of the herbal solution by gavage,respectively.The blank and model groups received an equal volume of 0.1％sodium carboxymethylcellulose solution by gavage,once daily for 30 consecutive days.The body weight changes of rats during the administration period were recorded.Calculate the prostate wet weight index.Prostate tissues were examined using hematoxylin and eosin(HE)staining,and the serum levels of cyclooxygenase-2(COX-2),prostaglandin E2(PGE2),interleukin-6(IL-6),interleukin-1β(IL-1β),monocyte chemoattractant protein-1(MCP-1),and nerve growth factor(NGF)were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the blank group,the model group showed no significant difference in body weight.In the model group,prostate structure was disrupted,and glandular epithelial atrophy and increased interstitial inflammatory cell infiltration were detected.Meanwhile,the serum levels of COX-2,PGE2,IL-6,IL-1β,MCP-1 and NGF were significantly elevated(P＜0.01),while the prostate wet weight index significantly decreased(P＜0.01).Compared with the model group,all treatment groups showed no significant difference in rat body weight.In the positive control group and the high-and medium-dose Compound Jinqiancao Granule groups,significant prostate tissue repair was observed,with mild glandular cavity damage,complete glandular contour,and remarkably reduced inflammatory cell infiltration.The serum levels of COX-2,PGE2,IL-6,IL-1β,MCP-1 and NGF significantly decreased in the positive control group and the high-dose Compound Jinqiancao Granules group(P＜0.05,P＜0.01).The medium-dose group exhibited significantly reduced serum levels of COX-2,PGE2,IL-6 and NGF(P＜0.05),while only the serum COX-2 level significantly decreased in the low-dose group(P＜0.05).In addition,the prostate wet weight index significantly increased in the positive control group as well as the high-and medium-dose Compound Jinqiancao Granules groups(P＜0.05,P＜0.01).Conclusion Compound Jinqiancao Granules exert a therapeutic effect on rats with chronic nonbacterial prostatitis,and the underlying mechanism may be related to the reduction of serum COX-2,PGE2,IL-6,IL-1β,MCP-1,and NGF levels,thereby inhibiting the inflammatory response.