棱术通脉丸干预小鼠颈动脉粥样硬化痰瘀互结证的机制研究OA

Objective To investigate the mechanism of Lengzhu Tongmai Pills(LZTM)in intervening carotid arterial atherosclerosis(CAS)in mice with phlegm and blood stasis syndrome.Methods Forty-five 6-week-old male apolipoprotein E-deficient(ApoE-/-)mice of SPF grade were divided into 6 groups:a normal diet group,a model group,a high-dose LZTM group,a medium-dose LZTM group,a low-dose LZTM group,or a control group using the random number table method.There were 5 animals in the normal diet group and 8 in each of the remaining groups.Except for the normal diet group,CAS models with phlegm and blood stasis syndrome were established in all other groups.The normal diet group and model group were administered distilled water via gavage.The high-dose,medium-dose,and low-dose LZTM groups were administered LZTM solution at 46.8 g/(kg·d),23.4 g/(kg·d),and 11.7 g/(kg·d),respectively.The control group was administered simvastatin at 13 g/(kg·d).The gavage volume for all groups was 0.1 ml/10 g,once daily.After 4 consecutive weeks of intervention,serum tumor necrosis factor-α(TNF-α)and C-reactive protein(CRP)levels were detected using enzyme-linked immunosorbent assay(ELISA).Histopathological changes in carotid arteries were observed via hematoxylin and eosin(HE)staining and Oil Red O staining,with lipid droplet areas calculated.Results Compared with the normal diet group,the model group exhibited significantly elevated serum TNF-α and CRP levels(P＜0.0001),with substantial inflammatory cell infiltration and plaque formation in the carotid intima-media,as well as significantly increased lipid droplet areas(P＜0.05).All dose LZTM groups and the control group showed decreased serum TNF-α and CRP levels(P＜0.0001).All treatment groups displayed alleviated carotid intima-media thickness,foam cell count,and lipid deposition to various extents.The high-dose LZTM group demonstrated reduced lipid droplet areas(P＜0.05).Compared with the medium-dose and low-dose LZTM groups,the high-dose group exhibited lower serum TNF-α and CRP expression(P＜0.0001).Conclusion LZTM can inhibit pro-inflammatory cytokine release and ameliorate CAS with phlegm and blood stasis syndrome in mice.