地黄明目饮调控IGF-1、VEGF表达治疗糖尿病视网膜病变大鼠的作用机制OA

Objective To investigate the mechanisms by which Dihuang Mingmu Decoction treats diabetic retinopathy in rats by regulating insulin-like growth factor 1(IGF-1)and vascular endothelial growth factor(VEGF)expression.Methods Ninety SD rats were divided into Ninety SD rats were divided into a blank group,a model group,a Western medicine group,low-,medium-,and high-dose Dihuang Mingmu Decoction groups according to the random number table method,with 15 rats in each group.Except for the blank group,a diabetic retinopathy rat model was constructed in the other groups by intraperitoneal injection of streptozotocin.After successful modeling,the low-,medium-,and high-dose Dihuang Mingmu Decoction groups were administered the corresponding Decoction via gavage at 10.4 g/kg,20.8 g/kg,and 31.2 g/kg,respectively.The Western medicine group was administered calcium dobesilate tablets at 0.2 g/kg via gavage.The blank group and the model group were both administered 0.9％normal saline at 0.2 g/kg via gavage.Intervention was administered once in the morning and once in the evening,for a total of 8 weeks.Then,fasting blood glucose level was measured using a disposable blood glucose meter test strip.Pathological morphological changes in retinal tissues were observed via hematoxylin-eosin(HE)staining.Transforming growth factor-β1(TGF-β1)mRNA was determined by real-time quantitative polymerase chain reaction(RT-qPCR).IGF-1 and VEGF protein expression levels were detected by immunohistochemistry.Results In terms of fasting blood glucose levels,they were increased in the model group after administration,as well as at 2,4,and 8 weeks after administration,compared with the blank group(P＜0.05).In contrast,they were decreased in the Western medicine group,along with the low-,medium-,and high-dose Dihuang Mingmu Decoction groups at 2,4,and 8 weeks after administration,compared with the model group(P＜0.05).They were decreased in the medium-and high-dose Dihuang Mingmu Decoction groups at 2,4,and 8 weeks after administration,compared with the low-dose Dihuang Mingmu Decoction group(P＜0.05).Similarly,they were decreased in the high-dose Dihuang Mingmu Decoction group at 2,4,and 8 weeks after administration,compared with the medium-dose Dihuang Mingmu Decoction group(P＜0.05).As for TGF-β1 mRNA,IGF-1,and VEGF expression in retinal tissues at 8 weeks after administration,compared with the blank group,the model group exhibited increased expression(P＜0.05).Compared with the model group,the Western medicine group showed increased TGF-β1 mRNA expression,along with decreased IGF-1 and VEGF expression(P＜0.05).Compared with the Western medicine group,the medium-and high-dose Dihuang Mingmu Decoction groups demonstrated decreased expression(P＜0.05).Compared with the low-dose Dihuang Mingmu Decoction group,the medium-and high-dose Dihuang Mingmu Decoction groups displayed decreased expression(P＜0.05).Similarly,compared with the medium-dose Dihuang Mingmu Decoction group,the high-dose Dihuang Mingmu Decoction group exhibited decreased expression(P＜0.05).Conclusion Dihuang Mingmu Decoction exerts therapeutic effects on diabetic retinopathy model rats by regulating IGF-1 and VEGF expression.