糖肾灌肠方通过抑制TGF-β1/Smad通路改善糖尿病肾病肾脏纤维化的机制研究OA

Objective:To explore the protective effect of the Tangshen enema formula on renal fibrosis in diabet-ic kidney disease(DKD)and its underlying mechanism.Methods:Forty adult C57BL/6J mice were divided into 4 groups:normal control group(10 mice),model group(10 mice),telmisartan group(10 mice),Tangshen Enema for-mula group(10 mice).Blood glucose,blood creatinine and urine microalbumin were measured,kidney index(KI)was calculated.Renal tissues were collected for light microscopy and electron microscopy observation.Western blot was used to detect the protein expression of transforming growth factor β1(TGF-β1),Smad3 and fibronectin(FN).RT-qPCR was used to detect the mRNA of TGF-β1,Smad3 and FN.The differences of the above indicators among the groups were compared.Results:The study found that compared with the normal control group,the levels of blood creatinine,urine microalbumin,KI,protein expression and mRNA of TGF-β1,Smad3 and FN in the model group were significantly increased(all P＜0.05),the renal tissue of the model group showed significant fibrotic pathological changes;Compared with the model group,the levels of blood creatinine,urine microalbumin,KI,the protein expres-sion and mRNA of TGF-β1,Smad3 and FN in the Tangshen Enema formula group were significantly decreased(all P＜0.05),the degree of renal fibrosis in the Tangshen Enema formula group was significantly alleviated.Conclusion:The study indicates that the Tangshen Enema formula improves the degree of renal fibrosis in DKD mice by regulating the TGF-β1/Smad3/FN signaling pathway.