石芦清金颗粒调节IL-6/JAK2/STAT3通路对急性肺损伤小鼠炎症反应及氧化应激机制的影响OA

Objective:To investigate the preventive and protective mechanisms of Shilu Qingjin granule on li-popolysaccharide induced acute lung injury(ALI)in mice by regulating the Janus kinase 2/transcriptional signaling transducer and activator 3(JAK2/STAT3)signaling pathway.Methods:Male C57BL/6 mice were randomly divided into 7 groups:blank group,model group,Lianhua Qingwen granule positive control group,methylprednisolone tablet positive control group,Shilu Qingjin granule low-dose group,Shilu Qingjin granule medium dose group,and Shilu Qingjin granule high-dose group,with 8 mice in each group.Each group was given the same volume of 0.9％sodium chloride solution,corresponding concentrations of Shilu Qingjin granule,Lianhua Qingwen granule,and methylpred-nisolone by gavage.After 7 days of administration,an ALI model was established by tracheal instillation of lipopo-lysaccharide.Observed the general condition of each group of mice;HE staining method was used to observe patho-logical changes in lung tissue;ELISA method was used to detect the levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in mouse serum;Western blot was used to detect the protein expression of JAK2,p-JAK2,STAT3,p-STAT3,and IL-6 in mouse lung tissue.Immunofluorescence assay was used to measure the levels of reac-tive oxygen species(ROS)in mouse lung tissue.Results:Compared with the blank group,typical ALI pathological changes such as infiltration of inflammatory cells and destruction of alveolar structural integrity were observed in the lung interstitium of the model group mice.The levels of TNF-α and IL-6 in the serum were significantly increased(P＜0.01),and the expression levels of p-JAK2 and p-STAT3 proteins were significantly increased(P＜0.01);Compared with the model group,the positive control group of methylprednisolone tablets,the positive control group of Lianhua Qingwen granule,and the low,medium,and high dose groups of Shilu Qingjin granule showed significant improvement in lung tissue structure damage,reduced inflammatory cell infiltration,significantly decreased TNF-αand IL-6 levels(P＜0.01),decreased p-JAK2/p-STAT3 expression(P＜0.05),and decreased ROS levels(P＜0.05).With the increase of Shilu Qingjin granule dosage used for intervention treatment in mice,the protein expression lev-els of p-JAK2 and p-STAT3 showed a significant downward trend(P＜0.01).Conclusion:Shilu Qingjin granule have a protective effect on ALI mice,and its mechanism may be related to the regulation of the JAK2/STAT3 signaling pathway.