茄属植物人参果和番茄原生质体的分离与融合技术研究OA

Solanum muricatum is a characteristic and emerging fruit crop experiencing rapid development in Yunnan,and the creation of novel varieties is crucial for the industrial advancement.Protoplast fusion technology serves as an effective approach to achieve interspecific hybridization.To establish an efficient protoplast isolation and fusion system between Solanum muricatum and Solanum lycopersicum,this study utilized S.muricatum Yuanguo No.2 and S.ly-copersicum Jiali as experimental materials.For protoplast isolation,an enzymatic hydrolysis method was employed,focusing on the selection of explants,optimization of enzyme combinations,and determination of hydrolysis duration.For protoplast fusion,the Polyethylene Glycol(PEG)method was adopted,screening suitable conditions based on PEG 6000 concentration and treatment duration.The optimal isolation system for S.muricatum Yuanguo No.2 involved us-ing callus induced from sterile leaves as the explant,with an enzyme solution consisting of 1%cellulase+0.5%pecti-nase+0.1%hemicellulase+0.1%macerozyme,hydrolyzed for 12 hours.Under the conditions,the protoplast yield reached 5.09×105 protoplasts/g,with a viability of 71.71%.Conversely,for S.lycopersicum'Jiali',the optimal system utilized sterile leaves as the explant,with an enzyme solution of 1%cellulase+0.5%pectinase,hydrolyzed for 14 hours,yielding 12.37×105 protoplasts/g with a viability of 73.97%.Regarding protoplast fusion,the highest fusion rate(2.26%)was achieved when protoplasts from both species were treated with 35%PEG 6000 for 30 minutes.This study estab-lished the optimal source tissues,enzyme combinations,and digestion durations for protoplast isolation from S.muri-catum Yuanguo No.2 and S.lycopersicum Jiali.Furthermore,the appropriate PEG 6000 concentration and fusion dura-tion for protoplast fusion were preliminarily determined.The findings would provide technical support for advancing somatic hybridization between the two species.