血叶兰种质资源表型评价及非共生萌发研究OA

The study evaluated the phenotypic traits of 11 different germplasm accessions of Ludisia discolor,observing qua-litative and quantitative traits such as leaf color,vein type,plant height,stem diameter,leaf size,and inflorescence characte-ristics to establish an efficient in vitro regeneration system for L.discolor.Germplasm H-1(Meizhou,Guangdong)exhibited outstanding performance in plant height,stem internode length,leaf length,and leaf width,while germplasm 1-21(hybrid offspring from Bawangling)demonstrated significant advantages in stem diameter and the number of flowers.Germplasm 70(hybrid offspring from Bawangling)showed the largest flower size,while germplasm 89(hybrid offspring from Ba-wangling)exhibited the longest inflorescence peduncle and rachis.The superior germplasm 1-21 was selected and self-pollinated,and undehisced capsules collected after pollination were used as explants.Through asymbiotic germination and in vitro culture,the optimal culture media formulations for each stage were screened.In addition,substrate screening for acclimatization and transplanting was conducted.Further research revealed that the optimal seed germination medium was 1/2 MS+1.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L potato,with a germination rate of 83.67%.For adventitious bud pro-liferation,MS+3.0 mg/L 6-BA+0.1 mg/L NAA was the most effective,with a proliferation coefficient of 4.45.For rooting and strong seedling cultivation,1/2 MS+0.1 mg/L NAA+30 g/L banana was the optimal treatment,significantly increasing root number,fresh mass per plant,and leaf number.The highest survival rate during acclimatization was achieved using sphagnum moss as the sole substrate(98.89%),followed by pure pine bark(95.06%)and a mixture of sphagnum moss and pine bark(96.82%).This study established an efficient in vitro regeneration system for L.discolor,encompassing germplasm evaluation,asymbiotic seed germination,adventitious bud proliferation,seedling strengthening and rooting,and domestication and transplantation.The optimal culture media for each stage were identified,providing a theoretical basis and technical support for the large-scale propagation and germplasm innovation of elite L.discolor accessions.