132份黄皮种质资源农艺性状遗传多样性分析及综合评价OA

It was aimed to explore the genetic diversity of agronomic traits such as fruits and leaves of seed-grown wampee germplasms on Donghai Island,Zhanjiang,Guangdong,to screen germplasm with excellent comprehensive traits,and to provide theoretical basis and material support for effective preservation,innovative utilization,and variety breeding of wampee germplasm resources in this region.132 wampee resources were collected from Donghai Island as experimental materials.21 agronomic traits including single fruit weight,fruit shape index,single fruit seed weight,seed number,soluble solids content,and edibility rate were measured.Correlation,principal components,and clustering analyses were conducted to systematically and comprehensively evaluate the genetic diversity of local germplasm.Among the 132 wampee germplasms,the diversity index for 21 agronomic traits ranged from 1.71 to 2.05,with coef-ficients of variation ranging from 10.32%to 73.90%.The fruit morphology and quality traits,sugar acid components,and leaf traits of wampees all showed a normal or nearly normal distribution,indicating a rich diversity among dif-ferent wampee germplasms.Among them,D-malic acid content,oxalic acid content,seed number,seed coat weight,leaf area,ascorbic acid content,and single fruit seed weight showed significant variation,indicating significant po-tential for genetic improvement.The coefficients of variation for fruit shape index,transverse diameter,and longitu-dinal diameter were small.Correlation analysis revealed a complex network of associations among fruit size,soluble sugar content,and organic acid content.Cluster analysis divided the germplasms into 8 groups.Group Ⅶ contained 3 non-nucleated germplasms,which possessed the excellent trait of the highest single-fruit weight(10.44 g),making them highly promising unique germplasms for development.The cumulative contribution rate of the first six principal components extracted from principal component analysis reached 78.024%,and a comprehensive scoring model was constructed based on this.Using the components,a comprehensive scoring model was developed,which ultimately resulted in the selection of eight germplasm lines with outstanding comprehensive traits,86,88,82,106,63,61,39 and 80.The seed-grown wampee germplasm resources have rich genetic diversity.The seedless large-fruited group and eight excellent germplasms selected would provide important parental material for breeding high-yield,high-quality new wampee varieties.