台湾金线莲内根-贝壳杉烯合酶基因克隆及红光诱导表达OA

Ent kaurene synthase(KS)is a key rate-limiting enzyme in gibberellins(GAs)synthesis.The expression of the KS gene in Anoectochilus formosanus(A.formosanus)influences GAs synthesis and plant growth.In this paper,the AfKS gene open reading frame spans 2169 bp,encoding 723 amino acids.The AfKS gene expression was upregulated under red light stress,peaking at 42.38-fold higher than the control after 24 h.However,GAs were downregulated,with significant reductions in GA9,GA7,GA19 and GA8.Under red light conditions,the relative chlorophyll content in A.formosanus decreased(0.85 times),while MDA content increased(1.32 times).Nevertheless,red light stress elevated polysaccharide,total flavonoid,and free amino acid content by 1.13,1.02 and 1.34 times,respectively.After exogenous GAs added,relative chlorophyll content increased,MDA levels reduced,and polysaccharides,total flavonoids,and free amino acids elevated.This study would provide insights for further elucidating the mechanism of A.formosanus regu-lating GAs synthesis through KS under red light stress,thereby influencing plant stress resistance and secondary me-tabolite synthesis.