营养盐限制诱导的龙须菜卷曲分枝发育调控机制研究OA

In response to the abnormal branching of Gracilariopsis lemaneiformis observed in recent years in cultivation areas,this study investigated whether nutrient limitation could induce curly branch formation and preliminarily explored the mechanisms underlying this phenomenon.Thalli were cultured under different nitrogen and phosphorus levels,and changes in branch morphology were recorded.The induced curled branches were then characterized through morphological observation,physiological and biochemical measurements,and gene expression analysis.The results demonstrated that specific low-ni-trogen and low-phosphorus conditions consistently induced curly branch formation.This curled pheno-type was accompanied by disorganized cellular arrangement,reduced photosynthetic efficiency,and in-creased activities of oxidoreductase-related enzymes.Transcriptome analysis revealed significant down-regulation of photosynthesis-related pathways.Concurrently,up-regulation occurred in energy metabo-lism and oxidoreductase-related pathways.Taken together,these findings establish that nutrient limita-tion may affect branch development by altering photosynthesis,energy metabolism,oxidative status,and ROS-related signaling.These findings establish a mechanistic framework for elucidating branch de-velopment under nutrient limitation.