羊栖菜雌、雄生殖器官附生菌多样性与代谢调控差异研究OA

To explore the differential characteristics of epiphytic bacteria in female and male organs of Sargassum fusiforme,this study analyzed community diversity and metabolic function differences of the epiphytic bacteria on the reproductive receptacles and gas vesicles of Sargassum fusiforme by using 16 S rRNA high-throughput sequencing technology.The results showed that there were significant differ-ences in the epiphytic bacteria between male and female reproductive organs of Sargassum fusiforme.In terms of community diversity,the α diversity of the epiphytic bacteria on the female reproductive recep-tacles was higher than that on the male ones.β diversity analysis indicated that gender was the main fac-tor driving the structural differentiation of the epiphytic bacteria on the reproductive receptacles.Re-garding community composition,Proteobacteria and Planctomycetota were the dominant bacterial phyla in the reproductive organs of Sargassum fusiforme.The relative abundance of Planctomycetota was higher in the epiphytic bacteria of the female reproductive receptacles and gas vesicles,while Proteobac-teria dominated in the epiphytic bacteria of the male reproductive receptacles and gas vesicles.At the ge-nus level,the dominant epiphytic bacterial genera on the female reproductive receptacles were Hellea,Vibrio,and Blastopirellula,while male reproductive receptacles and gas vesicles were primarily domi-nated by Granulosicoccus and Litorimonas.Metabolic pathway analysis revealed that epiphytic bacteria on female reproductive receptacles exhibited significant upregulation in pathways such as glycosamin-oglycan degradation and steroid hormone biosynthesis.In contrast,epiphytic bacteria on male recepta-cles showed enrichment in carotenoid biosynthesis and oxidative phosphorylation pathways.Compared to male gas vesicles,epiphytic bacteria of the female gas vesicles significantly upregulated pathways in-cluding β-lactam resistance,glycosaminoglycan degradation,and sphingolipid metabolism.