基于16S rRNA扩增子序列分析艾草根际土壤微生物群落多样性和功能预测OA

To clarify the differences in bacterial community structure and diversity between rhizosphere and non-rhizosphere soils of Artemisia argyi,Illumina high-throughput sequencing combined with PICRUSt2 functional prediction were used to analyze microbial characteristics of A.argyi rhizosphere soil in Yuzhong,Gansu.The results showed that an average of 714,006 raw sequences with an average length of 417 bp were obtained,and the sample coverage was above 99.71％.The alpha-diversity indexes of Simpson,Shannon,Chao1,Observed species and Pielou_e were 0.99,9.96,32,418.0,32,206.5 and 0.86,respectively.Actinomycetota was the most dominant phylum in all samples and was significantly enriched in the rhizosphere of two-year-old A.argyi.The dominant phyla also included Pseudomonadota and Bacillota;the dominant orders were Micrococcales and Rhizobiales,and the dominant genera were Rhizobium,Lysobacte and Bacillus.Principal component analysis showed significant differences between rhizosphere and non-rhizosphere microbial communities,and A.argyi planting significantly improved the diversity and evenness of soil microbial community.Functional prediction indicated that the abundance of metabolism was the highest in KEGG level 1 pathways,and COG functions were mainly concentrated in membrane transport,translation,DNA replication,signal transduction,amino acid/carbohydrate metabolism and secondary metabolite synthesis.This study provides a theoretical basis for the prevention and control of continuous cropping obstacles and the regulation of rhizosphere microenvironment of A.argyi.