丁香油抑菌活性测定和缓释凝胶乳剂的创制OA

This study aimed to determine the inhibitory effect of clove oil on Botrytis cinerea.To address the thermodynamic instability and oxidative degradation of clove oil during storage,which compromise its antifungal efficacy,a clove oil sustained-release gel emulsion was prepared using the phase inversion method.This formulation leverages the favorable adhesion,thickening,emulsifying,suspending,and film-forming properties of gels,along with their safe and stable chemical characteristics.Suitable types and dosages of auxiliary materials including emulsifiers,cosolvents,antifreeze agents and gelling agents were screened via the phase inversion method.The appearance properties of the gel preparation,the release performance of clove oil in the sustained-release gel emulsion,the weight loss rate and swelling rate of the gel,as well as the biological activity of the sustained-release gel emulsion were evaluated.When the ratio of clove oil,emulsifier and co-emulsifier was 1∶3∶6,gellan gum was selected as the gelling agent,the contents of gelling agent and glycerol in the sustained-release gel were 1.2％and 9.0％,respectively,and the pH value was 5.5,all indicators passed the performance tests in the quality evaluation of the prepared clove oil sustained-release gel emulsion.The experimental results provide a reference for the formulation and application of clove oil.