棉蚜体色与体内细菌群落结构的关联性分析OA

[Objective]This study aims to elucidate the potential correlation between body color polymorphism and associated bacterial community structure in Aphis gossypii.[Methods]Full-length 16S rRNA gene sequencing of bacteria from green and yellow morphs of A.gossypii was performed using the PacBio sequencing platform.Operational taxonomic unit(OTU)clustering was conducted at 97％similarity level,followed by taxonomic annotation based on the Silva database(v138.2)using Blast and Bayesian algorithms.[Results]A total of 117 bacterial OTUs were identified in both green morph and yellow morph,belonging to 9 phyla,13 classes,35 orders,57 families,89 genera,and 114 species,with notable differences in bacterial composition between the two types.The green individuals harbored a greater diversity of bacteria(9 phyla,13 classes,34 orders,54 families,80 genera,and 100 species)compared to the yellow ones(6 phyla,9 classes,30 orders,47 families,75 genera,and 97 species).The classes of Acidobacteriia,Kapabacteria,Cyanophyceae,and Desulfuromonadia were unique to the green morph,encompassing 14 exclusive genera such as Verrucomicrobiaceae and Comamonas.No unique bacterial groups at the class level were identified in the yellow morph.Significant differences in bacterial relative abundance were observed between the two morphs.For instance,the relative abundance of Enterobacter was significantly higher in the yellow morph(2.39％)than that in the green morph(1.08％).Conversely,Pantoea was significantly more abundant in the green morph(1.45％)than that in the yellow morph(0.93％).Among the unique genera of the green morph,Comamonas had the highest relative abundance(1.02％),whereas Arsenophonus was the most abundant unique genus in the yellow morph(3.43％).[Conclusion]There are significant differences in the bacterial community composition between green and yellow morphs of A.gossypii.Analyses of key characteristics,including genus-level diversity,relative abundance,and the distribution of unique genera,indicate a certain correlation between color polymorphism and bacterial community composition in the cotton aphid.This study lays a solid foundation for further investigation into the mechanisms of microbial-mediated regulation of color differentiation in A.gossypii.