基于基因集富集分析阐释慢性阻塞性肺疾病有效方药抑制巨噬细胞炎症反应机制OA

Objective To explore the anti-inflammatory mechanism of regulating and tonifying lung and kidney formulae[in-cluding Bufei formula(补肺方),Bufei Jianpi Formula(补肺健脾方),Bufei Yishen Formula(补肺益肾方),Yiqi Zishen Formu-la(益气滋肾方)]for chronic obstructive pulmonary disease.Methods Macrophage inflammatory model was induced by lipopo-lysaccharide(LPS),and then RNA-seq analysis was performed.Macrophage-inflammatory related pathways were obtained using gene set enrichment analysis(GSEA),and normalized enrichment score(NES)was used to identify pathways that were re-versed by traditional Chinese medicine(TCM)treatment.Results Compared with those in the normal group,244 significantly dis-turbed pathways(FDR＜0.05)were identified in the macrophage-inflammatory group,which were mainly related with inflamma-tion,immunity and metabolism.More than 50％of the above identified pathways could be significantly reversed by Dilong(Pheretima),Yinyanghuo(Epimedii Folium),Houpo(Magnoliae Officinalis Cortex)and Renshen(Ginseng Radix et Rhizoma).The NES of Bufei Yishen Formula was-1377.23,the NES of Yiqi Zishen Formula was-1250.01,the NES of Bufei Jianpi For-mula was-1041.56,and the NES of Bufei Formula was-720.02.Transforming growth factor beta production pathways were re-versed in most TCM-treated groups.Conclusion The intensity of regulating and tonifying lung and kidney formulae that reversed macrophage-inflammatory related pathways was in order of Bufei Yishen Formula,Yiqi Zishen Formula,Bufei Jianpi Formula and Bufei Formula.Dilong(Pheretima),Yinyanghuo(Epimedii Folium),Houpo(Magnoliae Officinalis Cortex)and Renshen(Ginseng Radix et Rhizoma)are the hub Chinese medicines for inhibiting the inflammatory response.Transforming growth factor beta pro-duction might be the important pathways of these formulae for inhibiting macrophage-inflammation.