仿生矿化硒掺杂羟基磷灰石负载GelMA水凝胶复合体系促进牙周炎性骨缺损修复OA
A biomimetically mineralized selenium-doped hydroxyapatite-loaded GelMA composite hydrogel for repairing periodontitis-associated inflammatory bone defects
目的:构建仿生矿化硒掺杂羟基磷灰石/甲基丙烯酰化明胶(Se-HA/GelMA)复合水凝胶,评价其促进牙周炎性骨缺损修复的作用.方法:制备 Se-HA/GelMA 水凝胶并进行理化表征;体外检测其对小鼠胚胎成骨细胞前体细胞(MC3T3-E1)成骨分化、活性氧(reactive oxygen species,ROS)水平及小鼠巨噬细胞(RAW264.7)极化的影响;体内建立大鼠牙周炎性骨缺损模型,通过 Micro-CT、组织学及免疫荧光评价修复效果.结果:Se-HA/GelMA 具有良好成胶性能,可降低细胞及缺损区 ROS 水平,促进成骨相关指标表达,并调节巨噬细胞向 M2 型极化;体内可改善骨微结构并减轻局部炎症.结论:Se-HA/GelMA 复合水凝胶具有抗氧化、免疫调节及促成骨作用,可促进牙周炎性骨缺损修复.
Objective:To construct a Se-HA/GelMA composite hydrogel and evaluate its effect on the repair of peri-odontitis-associated bone defects.Methods:Se-HA/GelMA hydrogel was prepared and characterized.In vitro,its effects on osteogenic differentiation and reactive oxygen species(ROS)levels in MC3T3-E1 cells,as well as macrophage polarization in RAW264.7 cells,were evaluated.In vivo,a rat model of periodontitis-associated bone defects was established to assess bone repair and local inflammatory changes.Results:Se-HA/GelMA showed favorable gelation performance,reduced ROS levels,promoted osteogenesis-related marker expression,regulated macrophage polarization toward the M2 phenotype,and improved bone microstructure in the defect area.Conclusion:Se-HA/GelMA exhibits antioxidative,immunomodulatory,and pro-osteo-genic effects,and may promote the repair of periodontitis-associated bone defects.
刘宇晴;雷皓淇;陈莉莉
华中科技大学同济医学院附属协和医院口腔科 湖北 武汉 430022华中科技大学同济医学院附属协和医院口腔科 湖北 武汉 430022华中科技大学同济医学院附属协和医院口腔科 湖北 武汉 430022
医药卫生
硒掺杂羟基磷灰石GelMA水凝胶牙周炎氧化应激巨噬细胞极化骨修复
Selenium-doped hydroxyapatiteGelMA hydrogelPeriodontitisOxidative stressMacrophage polariza-tionBone regeneration
《临床口腔医学杂志》 2026 (9)
519-526,8
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