皱环球盖菇锰过氧化物酶基因家族及其在不同农业废弃物培养基上的表达OA

The manganese peroxidase(MnP)gene family plays a crucial role in the extracellular degradation of lignocellulose by white-rot fungi.In this study,the MnP gene family in the genome of Stropharia rugosoannulata was systematically identified and analyzed using bioinformatics approaches,and the effects of different agricultural wastes(T1-T5)on mycelial growth,MnP activity,and the relative expression levels of MnP gene family members were investigated.The results showed that eight MnP gene family members(SrMnP1-SrMnP8)were identified from the S.rugosoannulata genome,and they were distributed at six chromosomes.Phylogenetic analysis revealed species-specific clades.The addition of 2%cottonseed hull(T3)and 4%sawdust(T5)significantly promoted mycelial growth.The T5 treatment showed the highest mycelial dry weight,increasing 44 times as compared with the control.MnP activity was significantly increased in the sawdust treatments,with the highest enzyme activities observed in the 2%sawdust(T4)and 4%sawdust(T5)treatments,reaching 4.85 U/g and 4.25 U/g,respectively.Gene expression analysis revealed that SrMnP3 exhibited significantly higher expression levels as compared with other SrMnPs across all treatments.This study preliminarily revealed the composition and expression characteristics of the MnP gene family in S.rugosoannulata,defining SrMnP3 as a key player in lignocellulose degradation.These findings provide technical references for optimizing fungal degradation capabilities through molecular approaches and enhancing the resource utilization of agricultural and forestry residues.