变形菌门复合菌剂与厚壁菌门复合菌剂叶面喷施对当归生理代谢、产量及抗根腐病的影响OA
Effects of foliar spraying of Proteobacteria compound bacterial inoculant and Firmicutes compound bacterial inoculant on physiological metabolism,yield and root rot resistance of Angelica sinensis
本研究设置变形菌门复合菌剂(BL)、厚壁菌门复合菌剂(HH)及马铃薯葡萄糖肉汤(PDB)培养基(CK)3 个处理,通过叶面喷施后测定当归植株的光合特性、抗氧化酶活性、氮素代谢、激素水平、产量及药效成分等指标,系统评估两种复合菌剂的应用效果.研究结果表明,BL 处理和 HH 处理当归净光合速率、光合氮素利用效率显著高于对照(P<0.05).在抗氧化系统方面,BL 处理当归的 2,2′-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、过氧化物酶活性及总酚含量均显著高于对照(P<0.05).BL处理当归丙二醛含量显著低于对照(P<0.05).BL 处理当归中茉莉酸类物质、细胞分裂素类物质积累量较高.HH处理当归中生长素、水杨酸类物质积累量较高.BL 处理和 HH 处理当归中阿魏酸、阿魏酸松柏酯和 Z-藁本内酯含量均显著高于对照(P<0.05).BL 处理和 HH 处理当归不定根数、芦头直径、主根长、单株干质量显著高于对照(P<0.05).BL 处理和HH 处理当归根腐病发病率均显著低于对照(P<0.05).两种菌剂处理均能够促进当归植株的生长发育,有效增强当归的抗病性,实现促进生长提高质量的良好效果.
In this study,three treatments were set,including the Proteobacteria compound bacterial agent(BL),the Firmicutes compound bacterial agent(HH),and the potato dextrose broth(PDB)medium control(CK).After foliar spraying,the photosynthetic character-istics,antioxidant enzyme activities,nitrogen metabolism,hormone levels,yield and medicinal components of Angelica sinensis plants were determined to systematically evaluate the application effects of the two compound bacterial agents.The results showed that the net photosynthetic rate and photosyn-thetic nitrogen use efficiency of Angelica sinensis under BL and HH treatments were significantly higher than those of the control(P<0.05).In terms of the antioxidant system,the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacities,peroxidase activity and total phenol content of Angelica sinensis under BL treatment were significantly higher than those of the control(P<0.05).The malondialdehyde content of Angelica sinensis under BL treatment was significantly lower than that of the control(P<0.05).The accumulation of jasmonates and cytokinins in Angelica sinensis was higher under BL treatment,while the accumulation of auxins and sali-cylic acids was higher under HH treatment.The contents of ferulic acid,coniferyl ferulate and Z-ligustilide in Angelica sinensis under BL and HH treatments were significantly higher than those of the control(P<0.05).The number of adventi-tious roots,rhizome diameter,main root length and dry weight per plant of Angelica sinensis under BL and HH treatments were significantly higher than those of the control(P<0.05).The root rot incidence of Angelica sinensis under BL and HH treatments was significantly lower than that of the control(P<0.05).Both bacterial agent treatments could promote the growth and development of Angelica sinensis plants,effectively enhance the disease resistance of Angelica sinensis,and achieve favorable effects of growth promotion and quality improvement.
沙洁;杨涛;王琼;师立伟;赵东红;唐湛文;赵疆;党淑钟
甘肃省科学院生物研究所/甘肃省微生物资源开发利用重点实验室,甘肃 兰州 730000甘肃省科学院生物研究所/甘肃省微生物资源开发利用重点实验室,甘肃 兰州 730000岷县园艺指导站,甘肃 岷县 748400定西市经济作物技术推广站,甘肃 定西 743000岷县劲康中药材有限公司,甘肃 岷县 748400重庆市药物种植研究所/重庆市道地药材规范化生产工程技术研究中心,重庆 408435甘肃省科学院生物研究所/甘肃省微生物资源开发利用重点实验室,甘肃 兰州 730000甘肃省科学院生物研究所/甘肃省微生物资源开发利用重点实验室,甘肃 兰州 730000
生物科学
当归复合菌剂内源激素光合氮素利用效率产量品质
Angelica sinensiscompound bacterial agentendogenous hormonephotosynthetic nitrogen use effi-ciencyyieldquality
《江苏农业学报》 2026 (8)
1575-1583,9
甘肃省科学院重点研发项目(2025QYXQ-02)甘肃省自然科学基金项目(23JRRA1657)甘肃省科技特派员(团)专项(25CXNJ007)平凉市科技计划项目(PL-STK-2024A-025)
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