水稻果糖1,6-二磷酸酶调控对水稻条纹病毒和水稻黑条矮缩病毒的抗性OA

Rice stripe disease,caused by rice stripe virus(RSV),and rice black-streaked dwarf disease,caused by rice black-streaked dwarf virus(RBS-DV),are major viral diseases in rice production,posing a serious threat to high and stable yield.Breeding resistant rice varieties using plant resistance-related genes is an ef-fective strategy for controlling rice viral diseases;however,the mechanism underlying rice antiviral resistance remains to be further elucidated.Plant fructose-1,6-bisphosphatase(FBPase)plays a crucial role in the gluconeogenesis pathway and sucrose biosynthesis.Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)analysis showed that the expression of OscFBP3(gene accession number:LOC_Os03g16050)was suppressed in rice plants after RBSDV and RSV infection.Transient expression assay results in tobacco leaves revealed that the OscFBP3-YFP fusion pro-tein was localized to the cytoplasm,confirming that OscFBP3 encoded a cytoplasmic FBPase.In this study,oscfbp3 mutant plants were generated using clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9(CRISPR-Cas9)technology.Molecular identification results showed that oscfbp3#5 and oscfbp3#6 carried a single-nucle-otide insertion of"A"or"T"at the OscFBP3 target site(A in#5,T in#6),resulting in a translational frameshift and premature termination of protein translation.After inoculation of oscfbp3 mutants with RSV and RBSDV,virus resistance identification results showed that the mutant plants exhibited decreased resistance to the viruses,with a significantly higher disease incidence than wild-type plants.RNA and protein molecular detection results further demonstrated that virus accu-mulation levels were significantly higher in oscfbp3 mutant plants than in wild-type plants.Field agronomic trait survey re-sults showed that,compared with wild-type plants,the plant height and tiller number per plant of oscfbp3 mutant plants were significantly reduced.These findings indicate that OscFBP3 is a key gene regulating plant growth,development,and virus resistance.The findings of this study provide experimental data for further understanding antiviral mechanisms in rice and offer potential targets for enhancing rice variety resistance through the use of virus resistance-related genes.