LINC00852调节miR-671-5p/SETD5轴对食管鳞状细胞癌细胞恶性进展的影响OA
Effects of LINC00852 on malignant progression of esophageal squamous cell carcinoma cells by regulating the miR-671-5p/SETD5 axis
目的:探讨长链非编码RNA00852(LINC00852)调节微小RNA-671-5p(miR-671-5p)/含SET结构域蛋白5(SETD5)轴对食管鳞状细胞癌(ESCC)细胞恶性进展的影响.方法:RT-qPCR检测56例2023年3月—2024年3月期间本院手术切除的ESCC组织及癌旁组织中LINC00852、miR-671-5p、SETD5 mRNA表达.以人食管鳞状细胞癌细胞系TE11细胞为研究对象,将其分为对照组、sh-NC组、sh-LINC00852组、sh-LINC00852+anti-NC组、sh-LINC00852+anti-miR-671-5p组.EdU染色、Transwell实验、流式细胞术分别检测细胞增殖、迁移和侵袭、凋亡;免疫印迹检测细胞中增殖细胞核抗原(PCNA)、SETD5、基质金属蛋白酶9(MMP-9)、Bcl-2相关X蛋白(Bax)的蛋白表达;双荧光素酶报告基因实验检测LINC00852与miR-671-5p、miR-671-5p与SETD5之间的互作.结果:ESCC组织LINC00852与SETD5 mRNA表 达 高 于 癌 旁 组 织,miR-671-5p表 达 低 于 癌 旁 组 织.sh-LINC00852组EdU阳性细胞率、迁移数、侵袭数、LINC00852、SETD5 mRNA和蛋白、PCNA蛋白、MMP-9蛋白低于sh-NC组、对照组,凋亡率、miR-671-5p、Bax蛋白高于sh-NC组、对照组;与sh-LINC00852组、sh-LINC00852+anti-NC组相比,sh-LINC00852+anti-miR-671-5p组EdU阳性细胞率、迁移数、侵袭数、SETD5 mRNA和蛋白、PCNA蛋白、MMP-9蛋白表达升高,凋亡率、miR-671-5p、Bax蛋白表达降低.结论:LINC00852通过调节miR-671-5p/SETD5轴促进ESCC细胞的恶性进展.
Objective:To investigate the effect of long noncoding RNA00852(LINC00852)on the malignant progression of esophageal squamous cell carcinoma(ESCC)cells by regulating the microRNA-671-5p(miR-671-5p)/SET domain-containing protein 5(SETD5)axis.Methods:RT-qPCR was used to measure the expression levels of LINC00852,miR-671-5p,and SETD5 mRNA in the ESCC tissues and adjacent non-tumor tissues from 56 ESCC patients who underwent surgery in our hospital from March 2023 to March 2024.Human esophageal squamous cell carcinoma cell line TE11 cells were selected as research objects and divided into five groups:Control,sh-NC,sh-LINC00852,sh-LINC00852+anti-NC,and sh-LINC00852+anti-miR-671-5p.EdU staining,Transwell assay,and flow cytometry were applied to detect the effects of LINC00852 silencing on the proliferation,migration,invasion,and apoptosis of TE11 cells.Western blotting was employed to measure the expression of PCNA,SETD5,MMP-9,and Bax proteins in TE11 cells.Dual-luciferase reporter gene assay was utilized to determine the interaction between LINC00852 and miR-671-5p,and between miR-671-5p and SETD5.Results:The expression of LINC00852 and SETD5 mRNA in ESCC tissues was higher than that in adjacent non-tumor tissues,while the expression of miR-671-5p was lower.The EdU-positive cell rate,migration and invasion numbers,LINC00852,SETD5 mRNA and protein levels,PCNA and MMP-9 protein levels in the sh-LINC00852 group were lower than those in the sh-NC group and the Control group,while the apoptosis rate,miR-671-5p,and Bax protein levels were higher than those in the sh-NC group and the Control group.Compared with the sh-LINC00852 group and the sh-LINC00852+anti-NC group,the sh-LINC00852+anti-miR-671-5p group showed increased EdU-positive cell rate,migration and invasion numbers,SETD5 mRNA and protein levels,PCNA and MMP-9 protein expression,but decreased apoptosis rate,miR-671-5p expression,and Bax protein expression.Conclusion:LINC00852 promotes the malignant progression of ESCC cells by regulating the miR-671-5p/SETD5 axis.
许芬芬;刘劼;熊玉梅;郭剑锋;谢玉芳
攀钢集团总医院消化内科,攀枝花 617000攀钢集团总医院消化内科,攀枝花 617000攀钢集团总医院消化内科,攀枝花 617000攀钢集团总医院消化内科,攀枝花 617000攀钢集团总医院消化内科,攀枝花 617000
医药卫生
长链非编码RNA00852微小RNA-671-5p含SET结构域蛋白5食管鳞状细胞癌恶性进展
long noncoding RNA00852miR-671-5pSET domain-containing protein 5esophageal squamous cell carcinomamalignant progression
《解剖学杂志》 2026 (4)
355-361,7
攀枝花市2022年度市级指导性科技计划项目(2022ZD-S-48)
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