茯苓多糖调节IL-10/JAK1/STAT3信号通路对慢性萎缩性胃炎大鼠胃黏膜损伤的影响OA
Effect of Poria cocos polysaccharide on gastric mucosal injury in rats with chronic atrophic gastritis by modulating the IL-10/JAK1/STAT3 signaling pathway
目的:探索茯苓多糖调节白细胞介素(IL)-10(IL-10)/JAK1/STAT3信号通路对慢性萎缩性胃炎(CAG)大鼠胃黏膜损伤的影响.方法:SD大鼠随机分为对照组、模型组、茯苓多糖组、茯苓多糖+RO8191(JAK1/STAT3 信号通路激活剂)组、茯苓多糖+IL-10中和抗体组,以N-甲基-N'-硝基-N-亚硝基胍(MNNG)联合不规律饮食法构建CAG大鼠模型,分组及干预后测定大鼠胃液pH值,ELISA法测定血清胃状态相关指标与炎症因子水平;H-E染色观察胃黏膜组织学形态;免疫组织化学染色检测各组大鼠胃黏膜G细胞、D细胞数量;TUNEL染色检测各组大鼠胃黏膜组织细胞凋亡;免疫印迹检测各组大鼠胃黏膜组织IL-10/JAK1/STAT3信号通路相关蛋白表达.结果:与对照组比较,模型组大鼠胃黏膜形态产生病理损伤,胃液pH值、胃黏膜组织细胞凋亡指数、IL-8与IL-6水平、p-JAK1/JAK1与p-STAT3/STAT3升高,胃泌素与胃动素水平、G细胞与D细胞光密度值、血清及胃黏膜组织IL-10蛋白表达降低;与模型组比较,茯苓多糖组大鼠胃黏膜组织病理学形态损伤减轻,胃液pH值、胃黏膜组织细胞凋亡指数、IL-8与IL-6水平、p-JAK1/JAK1与p-STAT3/STAT3降低,胃泌素与胃动素水平、G细胞与D细胞光密度值、血清及胃黏膜组织IL-10蛋白表达升高;与茯苓多糖组比较,茯苓多糖+RO8191组、茯苓多糖+IL-10中和抗体组大鼠胃黏膜组织病理学形态损伤均加重,胃液pH值、胃黏膜组织细胞凋亡指数、IL-8与IL-6水平、p-JAK1/JAK1与p-STAT3/STAT3均升高,胃泌素与胃动素水平、G细胞与D细胞光密度值、血清及胃黏膜组织IL-10蛋白表达均降低.结论:茯苓多糖可减轻CAG大鼠胃黏膜损伤,可能是通过调节IL-10/JAK1/STAT3信号通路实现.
Objective:To explore the effect of Poria cocos polysaccharide on gastric mucosal injury in rats with chronic atrophic gastritis(CAG)and explore its potential mechanism involving the interleukin(IL)-10(IL-10)/JAK1/STAT3 signaling pathway.Methods:SD rats were randomly divided into the following groups:control,model,Poria cocos polysaccharide,Poria cocos polysaccharide+RO8191(a JAK/STAT3 signaling pathway activator),and Poria cocos polysaccharide+IL-10 neutralizing antibody.A CAG rat model was established by using N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)combined with irregular feeding.After intervention with Poria cocos polysaccharides,the pH value of rat gastric juice was measured.ELISA was applied to measure serum levels of gastric function-related indicators and inflammatory cytokines.H-E staining was performed to evaluate gastric mucosal histopathological change.Immunohistochemical staining was employed to detect the numbers of G cell and D cell in the gastric mucosa of rats.TUNEL staining was applied to detect apoptosis of gastric mucosal tissue cells of rats.Western blotting was adopted to detect the IL-10/JAK1/STAT3 signaling pathway-related proteins in the gastric mucosal tissues of rats.Results:Compared with the control group,the model group showed significant pathological damage in gastric mucosa,with increased gastric juice pH value,gastric mucosal tissue cell apoptosis index,IL-8 and IL-6 levels,and elevated p-JAK1/JAK1 and p-STAT3/STAT3 ratios,while gastrin,motilin,G cell and D cell optical density values,and serum and tissue protein expression of IL-10 were decreased.Compared with the model group,the Poria cocos polysaccharide group exhibited alleviated gastric mucosal damage,decreased gastric juice pH value,gastric mucosal tissue cell apoptosis index,IL-8 and IL-6 levels,and p-JAK1/JAK1 and p-STAT3/STAT3 ratios,along with increased gastrin,motilin,G cell and D cell optical density values,and IL-10 expression.Compared with the Poria cocos polysaccharide group,both the Poria cocos polysaccharide+RO8191 and the Poria cocos polysaccharide+IL-10 neutralizing antibody groups showed aggravated pathological damage to gastric mucosal tissue,increased gastric juice pH value,gastric mucosal tissue cell apoptosis index,IL-8 and IL-6 levels,and p-JAK1/JAK1 and p-STAT3/STAT3 ratios,while decreased gastrin,motilin,G cell and D cell optical density values,and IL-10 serum and protein expression.Conclusion:Poria cocos polysaccharide can alleviate gastric mucosal injury in CAG rats,possibly by modulating the IL-10/JAK1/STAT3 signaling pathway.
刘文辉;陈佳星;白璐璐
内蒙古医科大学第二附属医院药学部,呼和浩特 010030内蒙古医科大学第二附属医院药学部,呼和浩特 010030内蒙古医科大学第二附属医院药学部,呼和浩特 010030
医药卫生
茯苓多糖白细胞介素-10JAK1STAT3慢性萎缩性胃炎胃黏膜损伤
Poria cocos polysaccharideinterleukin-10JAK1STAT3chronic atrophic gastritisgastric mucosainjury
《解剖学杂志》 2026 (4)
344-349,400,7
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