罗哌卡因通过PKCε/TRPV1信号通路对大鼠分娩疼痛的影响OA

Objective:To explore the effects of ropivacaine(Rop)on labor pain in rats by regulating the protein kinase C epsilon(PKCε)/transient receptor potential vanilloid 1(TRPV1)signaling pathway.Methods:Pregnant rats were randomly divided into control(normal delivery,intrathecal catheter injection of 0.9%normal saline),L-Rop,M-Rop,and H-Rop(intrathecal catheter injection of 0.5,1.0,and 2.0 mg/kg Rop,respectively),and DCP-LA group(intrathecal catheter injection of 2 mg/kg Rop combined with intraperitoneal injection of 2.1 mg/kg DCP-LA,a PKCε/TRPV1 signaling pathway activator).The hot water tail-flick method was used to detect the threshold of labor pain.Sedation was assessed with the Richmond Agitation-Sedation Scale.ELISA was performed to detect serum levels of 5-hydroxytryptamine(5-HT),dynorphin(DYN),and glutathione(GSH).Immunohistochemical staining was adopted to measure brain-derived neurotrophic factor(BDNF)in spinal cord of rats.Western blotting was applied to detect PKCε/TRPV1 signaling pathway-related proteins in rat spinal cord.Results:Compared with the control group,the L-Rop,M-Rop,and H-Rop groups showed increased pain threshold,DYN,and GSH levels and decreased Richmond Agitation-Sedation Scale score,5-HT,BDNF,PKCε,and TRPV1 expression.Compared with the H-Rop group,the DCP-LA group exhibited decreased pain threshold,DYN,and GSH levels,and increased Richmond Agitation-Sedation Scale score,5-HT,BDNF,PKCε,and TRPV1 expression.Conclusion:Ropivacaine may alleviate labor pain in rats,which may be related to the inhibition of the PKCε/TRPV1 signaling pathway.