咪达唑仑减轻脑缺血/再灌注模型大鼠脑损伤OA

Objective To investigate the effect of midazolam(MDZ)on brain injury in rats with cerebral ischemia-reperfusion injury(CI/RI).Methods Rats with CI/RI were divided into five groups:CI/RI group,MDZ-L group(intravenous injection of 0.05 mg/kg MDZ),MDZ-M group(0.15 mg/kg MDZ)and MDZ-H group(0.30 mg/kg MDZ).Additionally,there was an MDZ-H+verteporfin(inhibitor of Hippo/YAP signaling pathway)group(intra-peritoneal injection of 10 mg/kg verteporfin).The control group received an equivalent volume of saline.Outcomes were evaluated by neurological deficits,infarct volume,histopathological changes in brain tissue,neuronal apopto-sis,inflammatory cytokines and related protein expression.Results The CI/RI group showed loosening of neuronal arrangement,enlarged cell bodies,and nuclear shift as compared to the control group.The brain tissue damage was less severe in the MDZ-L,MDZ-M,and MDZ-H groups as compared to the CI/RI group.The MDZ-H+verteporfin group exhibited more severe brain tissue damage than the MDZ-H group.The CI/RI group showed increased neurolog-ical deficit scores,infarct volume,level of TNF-α and IL-1β in brain tissue,and neuronal apoptosis rate as compared to the control group,while the expression of YAP and TAZ proteins in brain tissue was decreased(P＜0.05).The MDZ-L,MDZ-M,and MDZ-H groups showed a decrease in neurological deficits,infarct size,inflammatory cytokines and apoptosis rate,along with increased YAP and TAZ protein expression compared to the CI/RI group(P＜0.05).The MDZ-H+verteporfin group showed a reversal picture of these indicators(P＜0.05).Conclusions MDZ can par-tially activate the Hippo/YAP signaling pathway and reduce brain tissue damage.