LINC00339通过调控miR-15b-5p/E2F3轴促进人甲状腺癌细胞系的迁移和侵袭OA
LINC00339 promotes the migration and invasion of thyroid cancer cell lines by regulating the miR-15b-5p/E2F3 axis
目的 探讨长链非编码 RNA LINC00339 在甲状腺癌(TC)细胞迁移和侵袭中的作用及其分子机制.方法 将甲状腺癌细胞系 KTC-1 进行分组:sh-NC 组、sh-LINC00339 组、NC mimic 组、miR-15b-5p mimic 组、NC inhibitor组、miR-15b-5p inhibitor 组、sh-LINC00339+NCinhibitor 组、sh-LINC00339+miR-15b-5p inhibitor 组、miR-15b-5p mimic+pcDNA-NC 组、miR-15b-5p mimic+pcDNA-E2F3 组、sh-LINC00339+pcDNA-NC 组和 sh-LINC00339+pcDNA-E2F3 组.每组设置 3 个复孔.通过 RT-qPCR 检测 LINC00339、miR-15b-5p 和 E2F 转录因子 3(E2F3)mRNA 的表达水平;利用划痕实验和 Transwell 小室法评估细胞迁移和侵袭能力;瘤球形成实验检测干细胞特性;蛋白质免疫印迹检测细胞干性相关标志物的蛋白质表达.结果 与正常甲状腺细胞相比,TC 细胞系中 LINC00339 和 E2F3表达上调,miR-15b-5p 表达下调(P＜0.05).干扰 LINC00339 可降低 E2F3 表达,抑制细胞迁移、侵袭和干细胞特性,同时上调 miR-15b-5p.抑制 miR-15b-5p 可部分逆转这些效应(P＜0.05).过表达 miR-15b-5p 可抑制 E2F3表达和细胞迁移、侵袭及干细胞特性,而过表达 E2F3 则可恢复这些效应(P＜0.05).结论 LINC00339 在 TC 细胞系中呈高表达,可通过靶向下调 miR-15b-5p 表达,解除 miR-15b-5p 对 E2F3 的抑制作用,从而诱导 TC 细胞系的干细胞特性,进而促进细胞迁移和侵袭.
Objective To investigate the role of long non-coding RNA LINC00339 in the migration and invasion of thyroid cancer(TC)cells and its molecular mechanism.Methods Thyroid cancer cell line KTC-1 was divided into sh-NC group,sh-LINC00339 group,NC mimic group,miR-15b-5p mimic group,NC inhibitor group,miR-15b-5p inhibitor group,sh-LINC00339+NC inhibitor group,sh-LINC00339+miR-15b-5p inhibitor group,miR-15b-5p mimic+pcDNA-NC group,miR-15b-5p mimic+pcDNA-E2F3 group,sh-LINC00339+pcDNA-NC group and sh-LINC00339+pcDNA-E2F3 group.Three holes were set in each group.The expression levels of LINC00339,miR-15b-5p and E2F3 mRNA were detected by RT-qPCR.Wound healing assay and Transwell assay were used to evaluate the ability of cell migration and invasion.Tumor sphere formation assay was used to detect the characteris-tics of stem cells.Western blot was used to detect the protein expression of stemness-related markers.Results Compared with normal thyroid cells,the expression of LINC00339 and E2F3 in TC cell lines was up-regulated,and the expression of miR-15b-5p was down-regulated(P＜0.05).Interfering with LINC00339 can reduce the expres-sion of E2F3,inhibit cell migration,invasion and stem cell characteristics,and up-regulate miR-15b-5p.Inhibition of miR-15b-5p partially reversed these effects(P＜0.05).Over-expression of miR-15b-5p could inhibit E2F3 ex-pression and cell migration,invasion and stem cell characteristics,while over-expression of E2F3 could restore these effects(P＜0.05).Conclusions LINC00339 is highly expressed in TC cell lines,which can relieve the inhibi-tory effect of miR-15b-5p on E2F3 by targeting down-regulation of miR-15b-5p expression,thereby inducing the stem cell characteristics of TC cell lines and promoting cell migration and invasion.
张友梁;任光玉;吴嘉;朱皓皞
中国人民解放军联勤保障部队第九〇八医院 病理科,江西 南昌 330002中国人民解放军联勤保障部队第九〇八医院 病理科,江西 南昌 330002中国人民解放军联勤保障部队第九〇八医院 病理科,江西 南昌 330002中国人民解放军联勤保障部队第九〇八医院 病理科,江西 南昌 330002
医药卫生
LINC00339miR-15b-5pE2F转录因子3甲状腺癌细胞干性
LINC00339miR-15b-5pE2F transcription factor 3thyroid carcinomacell stemness
《基础医学与临床》 2026 (9)
1175-1182,8
联勤保障部队第908医院苗子基金(YNKT202414,YNKT2025002)
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