宁波近岸海域水体和沉积物中原核与真核生物多样性和分布特征OA

Marine microorganisms play a crucial role in driving material cycling within marine ecosystems and contributing to marine primary productivity.Since traditional marine biological survey methods often underestimate microbial community diversity,this study utilized high-throughput amplicon sequencing of 16S/18S ribosomal RNA(rRNA)genes to analyze the diversity of prokaryotes and eukaryotes in the water and sediments of Ningbo coastal waters(including four regions:Hangzhou Bay,Xiangshan Bay,eastern Xiangshan,and Sanmen Bay).The results showed that the species richness of prokaryotes was higher than that of eukaryotes in different habitats of Ningbo coastal waters,with the highest prokaryotic species richness observed in the sediments of Sanmen Bay.Proteobacteria was the absolutely dominant phylum in prokaryotes,while Dinoflagellata represented the most dominant phylum in eukaryotes.The results of the α-diversity indices indicated that there were significant differences in microbial community diversity across different regions in the water environment;Xiangshan Bay exhibited the highest diversity,while Hangzhou Bay had the lowest.In the sedimentary environment,differences among regions were relatively small.The β-diversity results showed that the community structure in the water of eastern Xiangshan differed significantly from that in other regions,whereas in the sediments,the community structure of Sanmen Bay differed significantly from those of Xiangshan Bay and Hangzhou Bay.MetagenomeSeq analysis indicated that the differences between Hangzhou Bay and other regions were primarily reflected in the abundance of SAR11 Clade Ⅰa:it was the lowest in the water of Hangzhou Bay,whereas it was the highest in the sediments of Hangzhou Bay.This study preliminarily characterized the spatial distribution of marine microbial resources in Ningbo coastal waters,which holds great significance for the exploitation and utilization of marine biological resources and the sustainable development of the marine ecological environment in this region.