枇杷花抗炎物质制备及其体外抗炎活性研究OA

To achieve the high-value utilization of loquat flowers,anti-inflammatory substances were extracted and separated using macroporous resins,and then their anti-inflammatory activity was evaluated in vitro.The results showed that the optimal separation conditions were achieved with DM-130 resin,using sequential elution with distilled water followed by 70％ethanol at a flow rate of 1 mL·min-1.High Performance Liquid Chromatography(HPLC)analysis indicated that the main components of anti-inflammatory preparation from loquat flower(LFAI-P)were hyperoside,rutin,quercetin,amygdalin,and astragalin.Among these,hyperoside and rutin were more abundant,with contents of 118.53 μg·mg-1 and 107.61 μg·mg-1,respectively.The anti-inflammatory activity of LFAI-P was assessed using a lipopolysaccharide(LPS)-induced inflammation model in RAW264.7 cells.It was found that LFAI-P with low dose(50 μg·mL-1)promoted the secretion of inflammatory cytokines such as nitric oxide(NO),interleukin-6(IL-6),interleukin-1 β(IL-1β),and tumor necrosis factor-α(TNF-α).However,LFAI-P with medium and high dose(100-200 μg·mL-1)significantly inhibited the release of these inflammatory cytokines.Western Blot analysis further showed that the expression of inducible nitric oxide synthase(iNOS)and the phosphorylation level of Nuclear Factor kappa-B p65 subunit(NF-κB p-65)in the medium and high dose groups were significantly decreased compared with those in the model group(P＜0.01).These results suggested that LFAI-P with medium and high dose exhibited significant anti-inflammatory effects.This study provided a theoretical basis for the deep processing of loquat flowers and the development of related health products.