涝害胁迫下不定根去除对甜瓜叶片生理生化及代谢组的影响OA
Effects of Adventitious Roots Removal on the Physiological,Biochemical,and Metabolomic Responses of Melon(Cucumis melo L.)Leaves under Waterlogging Stress
涝害是一种严重的非生物胁迫,致使受淹器官的氧气供应大幅降低.为应对低氧环境,甜瓜(Cucumis melo L.)幼苗的下胚轴会诱导形成不定根,然而关于不定根在涝害胁迫下的作用与功能研究相对匮乏.为了探明甜瓜幼苗在涝害胁迫下诱导形成的不定根的作用,本试验研究了人工去除不定根后,植株叶片在生理生化和代谢水平的变化.结果表明,不定根去除可导致植株叶片变黄、萎蔫等异常表型,与正常管理条件下的对照和仅受涝害胁迫的幼苗相比,去除不定根导致总叶绿素含量分别极显著降低63.08％和43.62％.此外,不定根去除后的丙二醛含量、H2O2含量、乙醇脱氢酶活性、超氧化物歧化酶活性和过氧化物酶活性均极显著增加,与对照和仅涝害胁迫处理相比,H2O2含量分别提高206.87％和33.81％,过氧化物酶活性分别提高289.19％和20.94％.代谢组分析发现WL vs CK、R-AR vs CK和R-AR vs WL比较组中分别鉴定到88、119和75个差异代谢物,其中8个代谢物为3个比较组所共有;类黄酮物质中的芍药苷3-(6″-乙酰半乳糖苷)、R-10-羟基华法林和葡萄素A,脂质物质中的布卢门醇C葡萄糖苷和16-羟基十六烷酸以及萜类物质中的巨大戟二萜醇在涝害胁迫下不定根去除后发挥重要作用.本研究结果为阐明涝害胁迫下不定根的功能及其响应机制提供了理论依据.
Waterlogging is a severe abiotic stress that markedly reduces oxygen availability to submerged organs.Under hypoxic conditions,the hypocotyls of melon(Cucumis melo L.)seedlings induce the formation of adventitious roots.However,the importance of these adventitious roots in melon under waterlogging stress remains largely underexplored.To investigate the role of adventitious roots induced by waterlogging stress in melon seedlings,the physiological,biochemical,and metabolic changes in leaves after artificial removal of these induced adventitious roots were studied.The removal of adventitious roots resulted in abnormal phenotypes,characterized as chlorotic and withered leaves.Compared with the seedlings under normal management and those subjected to waterlogging alone,the removal of adventitious roots led to a highly significant reduction in total chlorophyll content by 63.08％and 43.62％,respectively.Additionally,the removal of adventitious roots significantly elevated the levels of malondialdehyde,H2O2,alcohol dehydrogenase activity,superoxide dismutase activity,and peroxidase activity.Specifically,H2O2 levels increased by 206.87％and 33.81％,while peroxidase activity rose by 289.19％and 20.94％,compared to the normal control and intact waterlogging stress conditions,respectively.Through untargeted metabolomic analysis,a total of 88,119,and 75 differentially accumulated metabolites(DAMs)were identified in WL vs CK,R-AR vs CK and R-AR vs WL,respectively,with eight DAMs shared across all three comparison groups.Notably,peonidin 3-(6″-acetyl-galactoside),R-10-hydroxywarfarin and vitisidin A(annotated as flavonoids),blumenol C glucoside and 16-hydroxyhexadecanoic acid(categorized as lipids),and ingenol(classified as terpenoids),were identified as key metabolites that play important roles in the response to adventitious roots removal under waterlogging stress.This research provides a theoretical basis for elucidating the physiological functions and underlying response mechanisms of adventitious roots under waterlogging stress.
张焕欣;闫承璞;陈钱;张哲;李国权
江西省农业科学院园艺研究所,江西南昌 330200江西省农业科学院园艺研究所,江西南昌 330200江西省农业科学院园艺研究所,江西南昌 330200江西科技师范大学生命科学学院,江西南昌 330013江西省农业科学院园艺研究所,江西南昌 330200
农业科技
甜瓜涝害胁迫不定根活性氧代谢组
Cucumis melowaterlogging stressadventitious rootsreactive oxygen speciesmetabolome
《核农学报》 2026 (10)
1948-1959,12
江西省农业科学院基础研究与人才培养专项资助项目(JXSNKYJCRC202311),国家自然科学基金项目(32260756),中国博士后科学基金(2021M701514),江西省博士后科研择优资助项目(2021KY44)
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