马铃薯StKFB29基因克隆、亚细胞定位及其互作蛋白筛选与分析OA

Anthocyanins play a significant role in enhancing stress resistance and nutritional quality of potato.To investigate the anthocyanin metabolic mechanism in potato,we identified a potential regulatory gene,StKFB29,involved in anthocyanin metabolism through transcriptome sequencing of pigmented potato varieties in preliminary studies.To elucidate its function,the StKFB29 gene was successfully cloned in this study,and the subcellular localization of its encoded protein as well as its interacting proteins were analyzed.The results showed that the complete coding sequence(CDS)of StKFB29 is 1 137 bp in length,encoding 378 amino acids,and the protein contains one F-box domain and three Kelch motifs.Subcellular localization experiments confirmed that StKFB29 is localized in the nucleus.Using the yeast two-hybrid system,22 potential interacting proteins of StKFB29 were screened,and the expression patterns of genes encoding these interacting proteins were analyzed across different potato tissues.Furthermore,yeast two-hybrid and bimolecular fluorescence complementation assays confirmed the interactions between StKFB29 and phenylalanine ammonia-lyase(StPAL)as well as UDP-glucuronate decarboxylase 1(StUXS1).These findings provide a theoretical foundation for further deciphering the functions and mechanisms of StKFB29 in plant metabolism and offer important genetic resources for molecular breeding in potato.