小豆锌指转录因子VaPLATZ7在抗病中的功能分析OA

To identify potential rust resistance genes in adzuki bean,the expression profiles of VaPLATZ7 were comparatively analyzed between the resistant cultivar LJ01 and the susceptible cultivar BQH after inoculation with Uromyces vignae using quantitative real-time PCR(qRT-PCR).Compared with the uninoculated control,the expression level of VaPLATZ7 in LJ01 increased to 56.9-fold at 12 h post-inoculation(hpi)and remained significantly higher than the control across 24 to 48 h,whereas its expression in BQH was markedly suppressed throughout the same period.Cis-acting element analysis of the VaPLATZ7 promoter identified five methyl jasmonate(MeJA)-responsive motifs.Upon exogenous MeJA treatment,the gene was significantly induced within 2 h,peaking at 4.5-fold relative to the control.Moreover,transient over-expression of VaPLATZ7 in tobacco significantly enhanced plant resistance to Botrytis cinerea infection,resulting in a 15％reduction in lesion diameter compared with the control.Collectively,VaPLATZ7 positively regulates plant disease resistance which is likely modulated via the JA signaling pathway.These findings provide a candidate gene resource that can be further exploited for disease resistance breeding in adzuki bean.