黑麦NPC基因家族鉴定及苗期胁迫表达分析OA

Non-specific phospholipase C(NPC)is an important class of enzymes involved in plant phospholipid metabolism.It catalyzes the hydrolysis of phospholipids to generate diacylglycerol and phosphorylated head groups,thereby playing a crucial role in lipid signal transduction,membrane remodeling,and responses to abiotic stresses during plant growth and development.ScNPCs represent a group of genes family encoding NPC proteins in the genome of rye(Secale cereale L.).Comprehensive bioinformatics analyses were performed to evaluate their physicochemical properties,phylogenetic relationships,conserved motifs and domain structures,as well as cis-acting elements in promoter regions.In addition,their expression patterns were analyzed across different tissues,developmental stages,and under drought and low-temperature stress conditions.Results showed that six ScNPCs genes(ScNPC01-ScNPC06)are distributed across five rye chromosomes,encoding proteins ranging from 509-541 amino acids in length.Their promoter regions are enriched with cis-acting elements related to phytohormone signaling,stress responses,and developmental regulation.Transcriptomic analysis revealed that ScNPCs expressions exhibit pronounced spatiotemporal specificity,with all members being upregulated during post-pollination grain filling stage.Quantitative real-time PCR(qRT-PCR)further revealed that drought stress significantly induces the expression of ScNPC01-ScNPC06,whereas low-temperature stress selectively upregulates ScNPC04 at the middle phase of the treatment,while ScNPC02 and ScNPC03 show a transient rise followed by a subsequent decline.This study provides valuable candidate genes and a theoretical foundation for further functional studies on the NPC gene family in Secale cereale L.in response to diverse stress conditions.